Bcl-2 Antibody (PrecisionAbTM Antibody)

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Bcl-2 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with bcl-2 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 67 second exposure. Arrow points to bcl-2 (molecular weight 26 kDa)

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Bcl-2 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 2

Western blot analysis of COS-7 whole cell lysates probed with bcl-2 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/50,000, STAR207P) and visualized on the ChemiDoc MP with 300 second exposure

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  • Bcl-2 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
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  • Bcl-2 Antibody with Control Lysate
  • Mouse anti Bcl-2
(Rated 0.0 out of 5 based on 0 customer reviews)
    Bcl-2 antibody recognizes apoptosis regulator bcl-2. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00017WBdatasheet pdfdatasheet pdf100 µl
      VMA00017
      VMA00017KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00017KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human bcl-2 antibody recognizes the human apoptosis regulator bcl-2 oncoprotein, a 239 amino acid integral single pass membrane protein containing 4 BH motifs which lies within the cell rather than on the cell surface. The protein is localized to the outer mitochondrial membrane and plays a role in the inhibition of apoptosis.

        Mouse anti Human bcl-2 antibody reacts with small B lymphocytes in the mantle zone and many cells within T cell areas. Very few cells in germinal centres are stained. In the thymus many cells in the medulla are stained but the cortex shows weak or negative staining. In non-hematopoietic tissues few cells are stained (Krajewski et al. 1995). The antibody reacts with neoplastic cells of follicular lymphoma and anaplastic large cell lymphoma (Reed 1997).

        Mouse anti Human bcl-2 antibody recognizes bcl-2 as a ~26 kDa single band by western blotting in various human cell lysates and the COS-7 cell line.
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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