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Bcl-10 Antibody | 151

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Bcl-10 Antibody | 151 gallery image 1

Staining of paraffin embedded human tonsil with Mouse anti Human Bcl-10 (MCA2750GA) following antigen retrieval

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  • Bcl-10 Antibody | 151 thumbnail image 1
  • Mouse anti Human Bcl-10:Alexa Fluor®488
  • Mouse anti Human Bcl-10
  • Mouse anti Human Bcl-10:RPE
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  • Product Type
    Monoclonal Antibody
  • Clone
    151
  • Isotype
    IgG1
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2750GAE, F*, IP, P*, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA2750GA
    MCA2750PEF*datasheet pdfdatasheet pdf100 Tests
    MCA2750PE
    MCA2750A488F*datasheet pdfdatasheet pdf100 Tests/1ml
    MCA2750A488
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Bcl-10 antibody, clone 151 recognizes human B-cell lymphoma/leukaemia 10 (Bcl-10), a 31kD CARD (caspase recruitment domain) containing molecule that is involved in the regulation of apoptosis. Bcl-10 was originally identified through its direct involvement in t(1;14)(p22;q32) of mucosa-associated lymphoid tissue (MALT) lymphoma. The Bcl-10 molecule is predominantly located in the cytoplasm but expression has also been reported in the nucleus of some lymphomas.

      Reports suggest that Bcl-10 is essential for nuclear factor (NF)-kappaB activation after lymphocyte receptor antigen stimulation and for the development of mature B-cells via ubiquitination of NEMO (Zhouet al. 2004).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to Alexa Fluor 488 - liquid
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
    • Reconstitution
      Reconstitute with 1.0ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
    • Immunogen
      Full length recombinant Bcl-10.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.05 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the NS0 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
    • GO Terms
      GO:0042109
      lymphotoxin A biosynthetic process
      Related reagents
      GO:0006915
      apoptosis
      Related reagents
      GO:0002237
      response to molecule of bacterial origin
      Related reagents
      GO:0031625
      ubiquitin protein ligase binding
      Related reagents
      GO:0005634
      nucleus
      Related reagents
      GO:0045416
      positive regulation of interleukin-8 biosynthetic process
      Related reagents
      GO:0032765
      positive regulation of mast cell cytokine production
      Related reagents
      GO:0002906
      negative regulation of mature B cell apoptosis
      Related reagents
      GO:0032449
      CBM complex
      Related reagents
      GO:0051092
      positive regulation of NF-kappaB transcription factor activity
      Related reagents
      GO:0043123
      positive regulation of I-kappaB kinase/NF-kappaB cascade
      Related reagents
      GO:0051059
      NF-kappaB binding
      Related reagents
      GO:0043621
      protein self-association
      Related reagents
      GO:0008022
      protein C-terminus binding
      Related reagents
      GO:0045893
      positive regulation of transcription, DNA-dependent
      Related reagents
      GO:0031398
      positive regulation of protein ubiquitination
      Related reagents
      GO:0002224
      toll-like receptor signaling pathway
      Related reagents
      GO:0003713
      transcription coactivator activity
      Related reagents
      GO:0042226
      interleukin-6 biosynthetic process
      Related reagents
      GO:0045121
      membrane raft
      Related reagents
      GO:0005764
      lysosome
      Related reagents
      GO:0045087
      innate immune response
      Related reagents
      GO:0042327
      positive regulation of phosphorylation
      Related reagents
      GO:0043422
      protein kinase B binding
      Related reagents
      GO:0050852
      T cell receptor signaling pathway
      Related reagents
      GO:0005829
      cytosol
      Related reagents
      GO:0001843
      neural tube closure
      Related reagents
      GO:0051260
      protein homooligomerization
      Related reagents
      GO:0048471
      perinuclear region of cytoplasm
      Related reagents
      GO:0043130
      ubiquitin binding
      Related reagents
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat1/10
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunoprecipitation
      Western Blotting
      Flow Cytometry(1)
      Immunohistology - Paraffin(2)1/501/800
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
      (2)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Human tonsil
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
      MCA2750GA detects bands of approximately 32kDa and 37kDa in cell lysates.
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional Bcl-10 Antibody Formats

    Formats Clone Applications Sizes available
    Bcl-10 Antibody : Purified 151 E, F*, IP, P*, WB 0.1 mg
    Bcl-10 Antibody : Alexa Fluor® 488 151 F* 100 Tests/1ml
    Bcl-10 Antibody : RPE 151 F* 100 Tests
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF, WB
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:Alexa Fluor® 488MCA928A488100 Tests/1mlF
        MCA928A488
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:RPEMCA928PE100 TestsF
        MCA928PE

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Histar Detection SystemSTAR3000B150 TestsC, P
          STAR3000B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

              Human tonsil
              • Application NameReference Images
                Immunohistology - Paraffin
                Western Blotting

              References

              1. Ye, H. et al. (2005) MALT lymphoma with t(14;18)(q32;q21)/IGH-MALT1 is characterized by strong cytoplasmic MALT1 and BCL10 expression.
              J Pathol. 205:293 – 301.
              2. Ye, H. et al. (2000) BCL10 expression in normal and neoplastic lymphoid tissue. Nuclear localization in MALT lymphoma.
              Am J Pathol. 157:1147-54.

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