BAX Antibody | 1F5-1B7

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BAX Antibody | 1F5-1B7 gallery image 1

Staining of paraffin embedded human placenta with Mouse anti Human BAX:Azide Free (MCA2960Z)

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  • BAX Antibody | 1F5-1B7 thumbnail image 1
  • Mouse anti Human BAX:Preservative Free
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  • Product Type
    Monoclonal Antibody
  • Clone
    1F5-1B7
  • Isotype
    IgG2b
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2960ZP *datasheet pdfdatasheet pdf0.1 mg
    MCA2960Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human BAX antibody, clone 1F5-1B7 recognizes human Apoptosis regulator BAX, also known as BAX or Apoptosis regulator BAX, Bax is a 192 amino acid ~21 kDa proapoptotic member of the Bcl-2 family. BAX accelerates programmed cell death by binding to and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. It also induces the release of cytochrome C , activation of CASP3 and thereby apoptosis. Defects in BAX are found in some cell lines from hematopoietic malignancies as T-cell acute lymphoblastic leukemia, Burkitt lymphoma, and plasmacytoma.

      Multiple potential isoforms of BAX generated by alternative splicing have been described. Mouse anti Human BAX antibody, clone 1F5-1B7 binds to the canonical α isoform, however binding to other potential isoforms β, γ, δ, ε, ζ, ψ, and σ, all of which demonstrate variation within the immunogen sequence, has not been evaluated.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein with a GST tag corresponding to amino acids 1 - 193 of human BAX.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin(1)110 ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional BAX Antibody Formats

    Formats Clone Applications Sizes available
    BAX Antibody : Preservative Free 1F5-1B7 P * 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG2b:HRPHCA038P0.1 mgE
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      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
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      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
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      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
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      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
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      Human anti Mouse IgG2b:RPEHCA038PE100 TestsF
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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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