HCA018 recognises Basonuclin 2, a putative transcription factor specific for skin keratinocytes. Basonuclin 2 is localised to the nucleus, and is highly expressed in testis, uterus and small intestine, along with skin keratinocytes. Recent data have shown Basonuclin 2 to co-localize with SC35, suggesting a possible function in nuclear processing of mRNA.
N.B. Antibody reactivity and working conditions may vary between species.
A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
This product is lyophilised.
Reconstitute with 0.2ml distilled water
Metal chelate affinity chromatography
Recombinant human basonuclin 2 fusion protein
Approx. Protein Concentrations
Antibody concentration 0.5mg/ml following reconstitution
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Prior to reconstitution store at +4oC. After reconstitution store at -20oC. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.