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BAK Antibody

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  • Goat anti Human BAK (N-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    OBT1398WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat anti Human BAK antibody recognizes an epitope within the N-terminal (NT) region of human BAK (also known as Bcl -2 homologous antagonist/killer), a widely expressed pro-apoptotic protein and member of the Bcl-2 family, which acts as an inducer and accelerator of apoptosis through interaction with mitochondrial voltage-dependent anion channels.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      RatExpected from Sequence
      MouseExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to human BAK (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      0.5% Bovine Serum Albumin
    • Immunogen
      Peptide sequence ASGQGPGPPRQE-C corresponding to the N-terminal region of BAK.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      release of cytochrome c from mitochondria
      metal ion binding
      pore complex
      protein heterodimerization activity
      activation of pro-apoptotic gene products
      regulation of protein homodimerization activity
      regulation of mitochondrial membrane potential
      regulation of protein heterodimerization activity
      establishment or maintenance of transmembrane electrochemical gradient
      identical protein binding
      regulation of mitochondrial membrane permeability
      mitochondrial outer membrane
      induction of apoptosis by intracellular signals
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.5 ug/ml1 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      OBT1398 detects a band of approximately 25 kDa in A431cell lysates.
    • Instructions For Use

    Additional BAK Antibody Formats

    Formats Applications Sizes available
    BAK Antibody : Purified WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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