BAG1 Antibody

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BAG1 Antibody gallery image 1

Western blot analysis of whole cell lysate from PC-3 human prostate cancer cells probed with Rabbit anti Human BAG1 (AHP1171) at 1(A) and 2(B) ug/ml

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BAG1 Antibody gallery image 2

Immunohistochemical staining of human lymph node with Rabbit anti Human BAG1 (AHP1171)

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  • BAG1 Antibody thumbnail image 1
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  • Rabbit anti BAG1 (N-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1171WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1171
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Human BAG1 antibody recognizes the N-terminal (NT) region of Bcl-2 associated athanogene-1 (BAG1), a co-chaperone protein which binds to and regulates the 70 kDa heat shock proteins (Hsp70/Hsc70). BAG1 has also been shown to interact with the kinase Raf1, a number of steroid and tyrosine kinase receptors, and the Bcl-2 family of proteins. It is thought to function as a molecular switch between cell growth and differentiation, and cell survival.

      The BAG1 gene encodes three different isoforms; a predominantly cytosolic form of ~36 kDa (BAG1S), a ~46 kDa cytosolic and nuclear form (BAG1M), and a predominantly nuclear form of ~50 kDa (BAG1L).

      BAG1 displays potent neuroprotective activity in vivo against stroke, and could be used for reducing brain injury during cerebral ischemia and neurodegenerative diseases. BAG1 may also promote chemoresistance, growth factor independence, and anchorage independent cell growth in tumour cells.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to BAG1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02%Sodium Azide
    • Immunogen
      14 amino acid peptide near the amino terminus of human BAG1.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting12ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1171 detects a band of approximately 35kDa in PC-3 cell lysate.
    • Instructions For Use

    Additional BAG1 Antibody Formats

    Formats Applications Sizes available
    BAG1 Antibody : Purified WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
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      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
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      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
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      Sheep anti Rabbit IgG:DyLight®680STAR36D680GA0.1 mgF, IF, WB
      STAR36D680GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              Further Reading

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