Goat anti BACE1 antibody recognizes human beta-site APP-cleaving enzyme 1, also known as beta- secretase 1, BACE1, Aspartyl protease 2, Asp2 or Memapsin-2. BACE1 is a 501 amino acid, the aminio terminal 45 residues constituting the signal and propeptide moieties, ~70 kDa aspartic protease.
BACE1 is involved in the cleavage of the amyloid precursor protein (APP) leading to the generation and extracellular release of soluble amyloid beta peptide (Lin et al. 2000). Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease (Vassar et al. 1999).
Serum - liquid
Antisera to human BACE1 (C-terminal) were raised by repeated immunisations of goats with highly purified antigen.
Synthetic peptide CLRQQHDDFADDISLLK corresponding to the C-terminal amino sequence 458-501 of human BACE.
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Immunohistology - Paraffin(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.