This item has been added to your cart.
ATG7 Antibody
Staining of formalin fixed, paraffin embedded human adrenal gland with Rabbit anti Human ATG7 (AHP1412)
Enlarge- Rabbit anti Human ATG7
-
Product TypePolyclonal Antibody
-
IsotypePolyclonal IgG
1 Formats Available
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
Summary
Secondary Antibodies
Negative Isotype Controls
Useful Reagents
Positive Controls
Histology Controls
More Images
References
Reviews
-
- Rabbit anti Human ATG7 antibody recognizes human Autophagy-related protein 7, known also as Ubitiquin Activating Enzyme-E1 like Protein, ATG7 and APG7, a 78 kDa cytoplasmic protein.
In mammalian cells, ATG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. Studies indicate that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death.
ATG7 is evolutionally conserved and exhibits high homology within mammalian species. It expressed in wide range of tissues including kidney, live, bone marrow and lymph nodes.
-
Intended Use
-
Target SpeciesHuman
-
Product FormPurified IgG - liquid
-
Reconstitution
-
Preparation
-
Antiserum PreparationAntiserum to human ATG7 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
-
Preservative Stabilisers0.01% Sodium Azide (NaN3)
-
ImmunogenSynthetic peptide sequence corresponding to amino acid sequence 558-569 of Human ATG7 protein.
-
Purity
-
Approx. Protein ConcentrationsIgG concentration 1.0mg/ml
-
Reagents In The Kit
-
Preparing The Antibody
-
Test Principle
-
Buffer SolutionPhosphate buffered saline
-
StorageStore at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. -
Shelf Life18 months from date of despatch.
-
GO Termspositive regulation of protein modification processautophagyprotein lipidationprotein homodimerization activityprotein transportAPG12 activating enzyme activitycytoplasmcellular membrane fusionubiquitin activating enzyme activity
-
UniProt
-
Entrez Gene
-
Acknowledgements
-
RegulatoryFor research purposes only
- This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Yes No Min Dilution Max Dilution ELISA 1/15000 1/75000 Western Blotting 1/500 1/2000 Immunohistology - Paraffin(1) 10ug/ml (1)This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.- Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended Protocol
- ELISA
- Immunohistology
- Histology Positive Control TissueHuman prostate
- Immunofluorescence
- Western Blotting
- Instructions For Use
Additional ATG7 Antibody Formats
| Formats | Applications | Sizes available |
|---|---|---|
| ATG7 Antibody : Purified | E, P *, WB | 50 µg |
- Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)
Recommended Secondary Antibody
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E, WB | STAR121B | |
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C, E, P, WB | 2AB02B | |
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F, IF, WB | STAR36D488GA | |
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F, IF | STAR36D549GA | |
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F, IF | STAR36D649GA | |
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F, IF, WB | STAR36D680GA | |
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F, IF, WB | STAR36D800GA | |
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | STAR121F | |
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C, F | STAR34B | |
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E, WB | STAR121P | |
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C, E, WB | STAR124P | |
| Sheep anti Rabbit IgG:HRP (Multi Adsorbed) | STAR54 | 1 mg | C, E, WB | STAR54 | |
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | STAR35A |
Recommended Negative Isotype Control
Useful Reagents
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025C | 50 ml | P | BUF025C | |
| TidyBlot™ Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* | STAR209P | |
| Histar Detection System | STAR3000B | 150 Tests | C, P | STAR3000B | |
| Antigen Retrieval Buffer, pH8.0 | BUF025A | 500 ml | P | BUF025A |
Recommended Positive Controls
Histology Controls
Human prostate
You may also be interested in...
Formerly known as AbD Serotec, a global supplier and manufacturer of antibodies, kits, and reagents.
Copyright © 2017 Bio-Rad Laboratories, Inc. All rights reserved
Copyright © 2017 Bio-Rad Laboratories, Inc. All rights reserved
Ordering
Payment methods we support:
- Invoice / Purchase Order
- Credit card
Follow us on
|
|
|
|
|
Also accessible on: |
||||
|
||||