ATG4B Antibody

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Staining of formalin fixed, paraffin embedded human heart with Rabbit anti Human ATG4B (AHP2098)

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  • Rabbit anti ATG4B
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2098P *, WBdatasheet pdfdatasheet pdf50 µg
    AHP2098
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti ATG4B antibody recognizes human Cysteine protease ATG4B, also known as AUT-like 1 cysteine endopeptidase, Autophagin-1, Autophagy-related cysteine endopeptidase 1 or Autophagy-related protein 4 homolog B. ATG4B is a 393 amino acid ~45 kDa cytoplasmic cysteine protease. ATG4B is expressed mainly in skeletal muscle tissue but also in brain, heart, liver and pancreas (Mariño et al. 2003).

      ATG4 is involved in cytoplasm to vacuole transport and is involved in the progress of autophagy (Scherz-Shouval et al. 2007).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      ZebrafishExpected from Sequence
      XenopusExpected from Sequence
      MouseExpected from Sequence
      RatExpected from Sequence
      ChickenExpected from Sequence
      FishExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to ATG4 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.05% Sodium Azide (NaN3)
      0.2% Gelatin
    • Immunogen
      Synthetic peptide corresponding to a portion of the amino acid sequence between 300-450 of human ATG4 – KLH conjugated.
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1 ug/ml3 ug/ml
      Immunohistology - Paraffin(1)10 ug/ml
      (1)
      This product requires antigen retrieval using heat treatment. Citrate buffer, pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human heart
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional ATG4B Antibody Formats

    Formats Applications Sizes available
    ATG4B Antibody : Purified P *, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Histar Detection SystemSTAR3000B150 TestsC, P
          STAR3000B
          Histar Detection SystemSTAR3000A50 TestsC, P
          STAR3000A
          Histar Detection SystemSTAR3000C500 TestsC, P
          STAR3000C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Human heart

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