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ATG1 Antibody

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Staining of formalin fixed, paraffin embedded human kidney with Rabbit anti Human ULK1 (AHP2214)

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  • Rabbit anti ATG1
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2214P, WBdatasheet pdfdatasheet pdf50 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti Human ATG1 antibody recognises Human Serine/threonine-protein kinase ULK1, also known as Autophagy-related protein 1 homolog, ATG1 or Unc-51-like kinase 1. ATG1 is a 1050 amino acid ~115 kDa, ubiquitously expressed cytoplasmic protein belonging to the serine/threonine protein kinase family.

      ATG1 is an autophagy related protein which is required for the formation of autophagosomes. ATG1 is the the target of the TOR kinase signalling pathway regulating autophagy and forms a complex with ATG13 and RB1CC1.

      ATG1 is strongly expressed in a wide range of adult tissues including heart, lung, kidney, brain, liver, pancreatic tissue and skeletal muscle.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to human ULK1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.2% Gelatin
    • Immunogen
      A synthetic peptide corresponding to an amino acid sequence within the region 320-370 of Human ULK1.
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      protein complex binding
      pre-autophagosomal structure
      regulation of autophagy
      autophagic vacuole
      protein serine/threonine kinase activity
      ULK1-ATG13-FIP200 complex
      ATP binding
      protein localization
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin10ug/ml
      Western Blotting13ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
      This product does not require protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
    • Histology Positive Control Tissue
      Human kidney
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional ATG1 Antibody Formats

    Formats Applications Sizes available
    ATG1 Antibody : Purified P, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          Histar Detection SystemSTAR3000A50 TestsC, P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP

          Recommended Positive Controls

            Histology Controls

              Human kidney
                • Product Images

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