ATF6 Antibody (PrecisionAbTM Antibody)

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ATF6 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with ATF6 antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 180 second exposure. Arrow points to ATF6 (molecular weight 84 kDa)

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  • ATF6 Antibody with Control Lysate
  • Goat anti ATF6
  • ATF6 Antibody with Control Lysate
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    ATF6 (aa437-449) antibody recognizes cyclic AMP-dependent transcription factor ATF-6 alpha, also known as cAMP-dependent transcription factor ATF-6 alpha, activating transcription factor 6 alpha or ATF6-alpha. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00107WBdatasheet pdfdatasheet pdf100 µl
      VPA00107
      VPA00107KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00107K
      VPA00107KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00107KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Goat anti Human ATF6 antibody recognizes activating transcription factor 6 alpha, also known as cAMP-dependent transcription factor ATF-6 alpha.

        Encoded by the ATF6 gene, this protein is a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, ATF6 is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. ATF6 has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in ATF6 with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. ATF6 is also thought to be a potential therapeutic target for cystic fibrosis (provided by RefSeq, Aug 2011).

        Goat anti Human ATF6 antibody detects a band of 84 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti ATF6 detects a band of approximately 84 kDa in HeLa cell lysates.
        Anti ATF6 detects a band of approximately 84 kDa in HeLa cell lysates.
        Anti ATF6 detects a band of approximately 84 kDa in HeLa cell lysates.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Goat polyclonal antibody purified by affinity chromatography
        Goat polyclonal antibody purified by affinity chromatography.
        Pack Size: 10 Westerns100μl Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Goat polyclonal antibody purified by affinity chromatography
      • Buffer Solution
        TRIS buffered saline
        TRIS buffered saline
        TRIS buffered saline
      • Preservative Stabilisers
        0.02% Sodium Azide (NaN3) 0.5 % BSA
        0.02% Sodium Azide (NaN3) 0.5 % BSA
        0.02% Sodium Azide (NaN3) 0.5 % BSA
      • Immunogen
        Peptide with the sequence C-NSYRYDHSVSNDK, from the internal region of the protein sequence
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HeLa lysate lyophilized in RIPA buffer.
        400μg HeLa lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        nuclear envelope
        nucleoplasm
        sequence-specific DNA binding transcription factor activity
        sequence-specific DNA binding
        RNA polymerase II transcription factor activity
        integral to membrane
        Golgi membrane
        positive regulation of gene-specific transcription involved in unfolded protein response
        protein dimerization activity
        transcription coactivator activity
        endoplasmic reticulum membrane
        protein folding
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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