ATF2 (pThr52) Antibody

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  • Rabbit anti ATF2 (pThr52)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2272WB*datasheet pdfdatasheet pdf0.1 ml
    AHP2272
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti ATF2 (pThr52) antibody recognizes activating transcription factor 2 (ATF2), also known as cyclic AMP-dependent transcription factor ATF-2 and cyclic AMP-responsive element-binding protein 2 (CREB-2), when phosphorylated at threonine 52.

      The ATF2 gene encodes a transcription factor that is a member of the leucine zipper family of DNA-binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. The protein forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. Several studies implicate abnormal activation of ATF-2 in growth and progression of mammalian skin tumors.

      ATF2 may mediate oncogenesis caused by mutant Ras protein and regulate maintenance of the aggressive cancer phenotype of some types of epithelial cells. ATF2 has also been shown to be phosphorylated by ATM at Serine 490 and 498 in humans. The serine phosphorylated forms play a role in mediating DNA repair and in response to DNA damage induced by ionizing radiation (IR).

      ATF2 exhibits histone acetyltransferase (HAT) activity and has been reported to specifically acetylate histones H2B and H4 in vitro. In concert with CUL3 and RBX1, ATF2 promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. This protein can also elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.

      In response to stress stimuli, ATF2 activates a variety of gene targets that are involved in oncogenesis, and has been correlated with maintenance of a cancer cell phenotype. Inhibiting ATF2 impedes melanoma development and elicits tumor suppressorfunction. To act as a tumor suppressor, ATF2 must localize at the mitochondria and phosphorylation at Thr52 by PKCε regulates this translocation.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      BovineExpected from Sequence
      ChickenExpected from Sequence
      MouseExpected from Sequence
      PrimateExpected from Sequence
      RatExpected from Sequence
      XenopusExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to ATF2 (pThr52) was raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      50% Glycerol
    • Immunogen
      Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr52 (pThr52) region of human ATF2
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      HEPES buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • GO Terms
      innate immune response
      MyD88-dependent toll-like receptor signaling pathway
      protein dimerization activity
      Toll signaling pathway
      zinc ion binding
      toll-like receptor 4 signaling pathway
      nucleoplasm
      regulation of transcription factor activity
      MyD88-independent toll-like receptor signaling pathway
      stress-activated MAPK cascade
      toll-like receptor 1 signaling pathway
      transcription coactivator activity
      toll-like receptor 3 signaling pathway
      sequence-specific DNA binding transcription factor activity
      toll-like receptor 2 signaling pathway
      sequence-specific DNA binding
      RNA polymerase II transcription factor activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting(1)1/250
      (1)
      For the detection of phosphoproteins, phosphatase inhibitors should always be added freshly to the lysis buffer. Milk or other casein-based blocking solutions are generally not recommended for blocking; as casein is itself a phosphoprotein its use can result in high background.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Rabbit anti Human ATF2 detects a band of approximately 72 kDa in UACC903 melanoma cell line lysate
    • Instructions For Use

    Additional ATF2 (pThr52) Antibody Formats

    Formats Applications Sizes available
    ATF2 (pThr52) Antibody : Purified WB* 0.1 ml
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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