ATF2 (pSer94/pSer112) Antibody

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ATF2 (pSer94/pSer112) Antibody gallery image 1

Western blot analysis of extracts from HUVEC cells untreated or treated with Anisomycin using Rabbit anti ATF2 (pSer94/pSer112) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with Anisomycin (5ug/ml) for 30 minutes

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ATF2 (pSer94/pSer112) Antibody gallery image 2

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rabbit anti ATF2 (pSer94/pSer112) antibody

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  • Rabbit anti ATF2 (pSer94/pSer112)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2551P, WBdatasheet pdfdatasheet pdf50 µl
    AHP2551
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti ATF2 (pSer94/pSer112) antibody recognizes activating transcription factor 2 (ATF2), also known as cyclic AMP-dependent transcription factor ATF-2 and cyclic AMP-responsive element-binding protein 2 (CREB-2), when phosphorylated at serine 94 or serine112. ATF2 is a member of the ATF family of transcription factors containing single bZIP and C2H2-type zinc finger domains. ATF2 plays a role in the regulation of genes involved in cell differentiation, growth and the response to stress and DNA damage.

      ATF2 binds to the cAMP responsive element (CRE), stimulating CRE- dependent transcription (Shuman et al. 1997). ATF2 also functions as a histone acetyltransferase (HAT), specifically acetylating histones 2B and 4 in vitro (Kawasaki et al. 2000). Serine 112 of ATF2 is reported to be a phosphorylation site.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin1/501/100
      Western Blotting1/5001/2000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
    • Instructions For Use

    Additional ATF2 (pSer94/pSer112) Antibody Formats

    Formats Applications Sizes available
    ATF2 (pSer94/pSer112) Antibody : Purified P, WB 50 µl
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

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          Recommended Positive Controls

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              Further Reading

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