ASS1 Antibody

//www.bio-rad-antibodies.com/human-ass1-antibody-ahp1669.html?RenderPageSpecific=1
ASS1 Antibody gallery image 1

Purchased this product? Why not be the first to submit a review and upload an image?

Enlarge
  • ASS1 Antibody thumbnail image 1
  • Goat anti Human ASS1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1669E, IP, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1669
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Goat anti human ASS1 antibody recognises human argininosuccinate synthetase-1, an ornithine (urea) cycle enzyme which catalyzes the penultimate step of the arginine biosynthetic pathway.

      Defects in ASS1 genes are responsible for the autosomal recessive disease known as citrullinemia type 1 (CTLN1), characterized by increased levels of urine and serum citrulline, and ammonia intoxication.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      MouseExpected from Sequence
      RatExpected from Sequence
      DogExpected from Sequence
      Bovineyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to ASS1 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      0.5% Bovine Serum Albumin
    • Immunogen
      Peptide sequence C-ENPKNQAPPGLYTKTQD from the internal region of ASS1 (NP_000041.2; NP_446464.1).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      ATP binding
      cytosol
      argininosuccinate synthase activity
      protein binding
      urea cycle
      arginine biosynthetic process
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/64000
      Immunoprecipitation
      Western Blotting0.010.03ug/ml
      Immunohistology - Paraffin(1)2.04.0ug/ml
      (1)
      This antibody requires heat-mediated antigen retrieval prior to staining paraffin sections. Citrated buffer, pH6.0, is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human kidney
    • Immunofluorescence
    • Western Blotting
      Goat anti human ASS1 detects a band of approximately 45kDa in human kidney cell lysates
    • Instructions For Use

    Additional ASS1 Antibody Formats

    Formats Applications Sizes available
    ASS1 Antibody : Purified E, IP, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Human kidney

              Write your review

              You may also be interested in...