ARPC5 Antibody (PrecisionAbTM Antibody)

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ARPC5 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with ARPC5 antibody followed by detection with Goat anti Rabbit HRP conjugated (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to ARPC5 (molecular weight 16 kDa)

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  • Rabbit anti ARPC5
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    ARPC5 antibody recognizes ARPC5, also known as actin-related protein 2/3 complex subunit 5.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00849WBdatasheet pdfdatasheet pdf100 µl
      VPA00849
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Rabbit anti Human ARPC5 antibody recognizes ARPC5, also known as actin-related protein 2/3 complex subunit 5.

        ARPC5 encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by ARPC5, the p16 subunit, has yet to be determined. Alternatively spliced transcript variants encoding different isoforms have been observed for ARPC5 (provided by RefSeq, Jul 2012).

        Rabbit anti Human ARPC5 antibody detects a band of 16 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Anti ARPC5 antibody recognizes a band of approximately 16 kDa in THP-1 cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
      • Preparation
        Rabbit polyclonal antibody purified by affinity chromatography on immunogen
      • Buffer Solution
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide
      • Immunogen
        KLH conjugated synthetic peptide corresponding to amino acids 1-30 of human ARPC5
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
      • Lysate Reconstitution
      • Storage
        Store undiluted at -20°C, avoiding repeated freeze thaw cycles
      • Shelf Life
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        cytoplasm
        cell projection
        structural constituent of cytoskeleton
        Arp2/3 protein complex
        actin cytoskeleton organization
        cellular component movement
        actin binding
        regulation of actin filament polymerization
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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