ARNT Antibody

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Western blot analysis of cell and tissue lysates using Rabbit anti ARNT antibody

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  • Rabbit anti ARNT
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2436WBdatasheet pdfdatasheet pdf50 µl
    AHP2436
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti ARNT antibody recognizes aryl hydrocarbon receptor nuclear translocator (encoded by the gene ARNT), also known as hypoxia-inducible factor 1-beta (HIF1-beta) or bHLHe2. ARNT is a member of the bHLH PAS (basic helix-loop-helix Per-ARNT-Sim) family of transcription factors. ARNT forms complexes with either hypoxia-inducible factor 1-alpha (HIF-1 alpha) or aryl hydrocarbon receptor (AhR), ARNT is involved in the adaptive response to stress caused by dioxins and hypoxia.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      RatExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to ARNT was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide
      50% Glycerol
    • Immunogen
      Recombinant human ARNT
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      signal transducer activity
      regulation of transcription from RNA polymerase II promoter in response to oxidative stress
      aryl hydrocarbon receptor binding
      positive regulation vascular endothelial growth factor production
      mRNA transcription from RNA polymerase II promoter
      response to hypoxia
      positive regulation of glycolysis
      positive regulation of erythrocyte differentiation
      positive regulation of hormone biosynthetic process
      positive regulation of vascular endothelial growth factor receptor signaling pathway
      positive regulation of endothelial cell proliferation
      protein heterodimerization activity
      transcription regulator activity
      sequence-specific DNA binding transcription factor activity
      transcription coactivator activity
      positive regulation of gene-specific transcription
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/5001/2000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Rabbit anti ARNT antibody detects a band of approximately 87 kDa in cell lysates under reducing conditions. Rabbit anti ARNT antibody also appears to detect a modified form of ARNT at a higher molecular weight.
    • Instructions For Use

    Additional ARNT Antibody Formats

    Formats Applications Sizes available
    ARNT Antibody : Purified WB 50 µl
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

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