ARFGAP3 Antibody

ARFGAP3 Antibody gallery image 1

Western blot analysis of ARFGAP3 in mouse brain extract probed with Goat anti Human ARFGAP3 (AHP697)

ARFGAP3 Antibody gallery image 2

Western blotting analysis of mouse brain extracts (35μg total protein in RIPA buffer) probed with Goat anti Human ARFGAP3 antibody (AHP697) at 2.0μgml-1. Primary incubated for 1 hour. Signal was detected by western blot using chemiluminescence

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  • Goat anti Human ARFGAP3
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP697WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat anti ARFGAP3 recognises ARFGAP3 (ADP - ribosylation factor GTPase activating protein 3), originally described as ARFGAP1.

      ARFGAP3 is thought to promote hydrolysis of ARF1 bound GTP, which is required for the dissociation of coat proteins from Golgi derived membranes and vesicles.

      ARFGAP3 is widely expressed, being found at high levels in endocrine glands and testis (Zheng et al. 2000).
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to ARFGAP3 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02%Sodium Azide
      0.5%Bovine Serum Albumin
    • Immunogen
      Synthetic peptide NGVVTSIQDRYGS derived from C terminus of ARFGAP3 protein.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      vesicle-mediated transport
      ARF GTPase activator activity
      zinc ion binding
      intracellular protein transport
      regulation of ARF GTPase activity
      protein transporter activity
      protein secretion
      Golgi membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1ug/ml3ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP697 detects a band of approximately 55-60kDa in mouse brain cell lysates. A minimum incubation time of 1 hour is recommended is recommended with this antibody.
    • Instructions For Use

    Additional ARFGAP3 Antibody Formats

    Formats Applications Sizes available
    ARFGAP3 Antibody : Purified WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls


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