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Apolipoprotein E antibody | WUE-4

Mouse anti Human Apolipoprotein E

Product Type
Monoclonal Antibody
Clone
WUE-4
Isotype
IgG1
Specificity
Apolipoprotein E

Product Code Applications Pack Size List Price Your Price Qty
MCA5639
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.2 mg loader
List Price Your Price
loader
MCA5639GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 mg loader
List Price Your Price
loader

Mouse anti Human Apolipoprotein E antibody, clone WUE-4 recognizes an epitope within amino acids 140-160 of human apolipoprotein E (Apo-E), a major component of very low-density lipoproteins (VLDLs). Apo-E is the principle apolipoprotein in the central nervous system, and is secreted by most organs into the plasma, playing a vital role in the binding, internalization and catabolism of triglyceride-rich lipoprotein constituents.

Apo-E acts as a ligand for both the specific apo-E receptor (chylomicron remnant) of hepatic tissues, and the apoB,E (LDL) receptor. Three isoforms of Apo-E have been identified, ApoE2, E3 and E4, and have been linked with various disorders. ApoE2 has been shown to bind LPL receptors with low affinity, resulting in increased plasma cholesterol and triglyceride levels, and thereby an increased risk in cardiovascular disorders. ApoE4 is a high risk factor for Alzheimers disease (Sanan et al. 1994), and in particular late onset Alzheimer disease 2 (AD2), whilst ApoE3 is the most common isoform, and considered the normal/natural Apo-E genotype.

Mouse anti Human Apolipoprotein E antibody, clone WUE-4 has been shown to inhibit Apo-E mediated binding of lipoproteins to the apoB,E cell receptor (Krul et al. 1998).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sea Lion
Harbour seal
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
MCA5639: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
MCA5639GA: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Purified ApoHDL fraction.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/O-Ag14 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/1000
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA5639 detects a major band of approximately 34-36kDa in human liver cell lysates.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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loader
Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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loader
Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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loader
Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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loader
Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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loader
Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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loader
Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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loader
Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Mouse IgG:FITC

Source Reference

  1. Krul, E.S. et al. (1988) Heterogeneity of apolipoprotein E epitope expression on human lipoproteins: importance for apolipoprotein E function.
    J Lipid Res. 29 (10): 1309-25.

References for Apolipoprotein E antibody

  1. Davis, R.W. et al. (1991) Lipoproteins in pinnipeds: analysis of a high molecular weight form of apolipoprotein E.
    J Lipid Res. 32 (6): 1013-23.
  2. Fagan, A.M. et al. (2004) ApoAI deficiency results in marked reductions in plasma cholesterol but no alterations in amyloid-beta pathology in a mouse model of Alzheimer's disease-like cerebral amyloidosis.
    Am J Pathol. 165: 1413-22.
  3. Fryer, J.D. et al. (2005) The low density lipoprotein receptor regulates the level of central nervous system human and murine apolipoprotein E but does not modify amyloid plaque pathology in PDAPP mice.
    J Biol Chem. 280 (27): 25754-9.
  4. Wahrle, S.E. et al. (2007) Apolipoprotein E levels in cerebrospinal fluid and the effects of ABCA1 polymorphisms.
    Mol Neurodegener. 2: 7.
  5. Hirsch-Reinshagen, V. et al. (2009) LCAT synthesized by primary astrocytes esterifies cholesterol on glia-derived lipoproteins.
    J Lipid Res. 50: 885-93.
  6. Fan, J. et al. (2011) An ABCA1-independent pathway for recycling a poorly lipidated 8.1 nm apolipoprotein E particle from glia.
    J Lipid Res. 52: 1605-16.
  7. Youmans, K.L. et al. (2011) Amyloid-β42 alters apolipoprotein E solubility in brains of mice with five familial AD mutations.
    J Neurosci Methods. 196: 51-9.
  8. Kim, J. et al. (2012) Anti-apoE immunotherapy inhibits amyloid accumulation in a transgenic mouse model of Aβ amyloidosis.
    J Exp Med. 209: 2149-56.
  9. View The Latest Product References
  10. Wildsmith KR et al. (2012) In vivo human apolipoprotein E isoform fractional turnover rates in the CNS.
    PLoS One. 7 (6): e38013.
  11. Jiang, J. et al. (2012) Hepatitis C virus attachment mediated by apolipoprotein E binding to cell surface heparan sulfate.
    J Virol. 86: 7256-67.
  12. Lee, C.Y. et al. (2012) Apolipoprotein E promotes β-amyloid trafficking and degradation by modulating microglial cholesterol levels.
    J Biol Chem. 287: 2032-44.
  13. Jiang, J. et al. (2013) Apolipoprotein e mediates attachment of clinical hepatitis C virus to hepatocytes by binding to cell surface heparan sulfate proteoglycan receptors.
    PLoS One. 8: e67982.
  14. Fu,Y. et al. (2016) Apolipoprotein E lipoprotein particles inhibit amyloid-β uptake through cell surface heparan sulphate proteoglycan.
    Mol Neurodegener. 11 (1): 37.

ELISA

Immunoprecipitation

Western Blotting

RRID
AB_10841622
UniProt
P02649
Entrez Gene
APOE
GO Terms
GO:0000302 response to reactive oxygen species
GO:0001540 beta-amyloid binding
GO:0001937 negative regulation of endothelial cell proliferation
GO:0007010 cytoskeleton organization
GO:0005543 phospholipid binding
GO:0006641 triglyceride metabolic process
GO:0006898 receptor-mediated endocytosis
GO:0006916 anti-apoptosis
GO:0006917 induction of apoptosis
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0007263 nitric oxide mediated signal transduction
GO:0007271 synaptic transmission, cholinergic
GO:0008201 heparin binding
GO:0008219 cell death
GO:0010544 negative regulation of platelet activation
GO:0010873 positive regulation of cholesterol esterification
GO:0010875 positive regulation of cholesterol efflux
GO:0016209 antioxidant activity
GO:0019934 cGMP-mediated signaling
GO:0030425 dendrite
GO:0030516 regulation of axon extension
GO:0030828 positive regulation of cGMP biosynthetic process
GO:0032488 Cdc42 protein signal transduction
GO:0032805 positive regulation of low-density lipoprotein particle receptor catabolic process
GO:0033344 cholesterol efflux
GO:0033700 phospholipid efflux
GO:0034361 very-low-density lipoprotein particle
GO:0034362 low-density lipoprotein particle
GO:0034363 intermediate-density lipoprotein particle
GO:0034364 high-density lipoprotein particle
GO:0034372 very-low-density lipoprotein particle remodeling
GO:0034375 high-density lipoprotein particle remodeling
GO:0034380 high-density lipoprotein particle assembly
GO:0034382 chylomicron remnant clearance
GO:0034384 high-density lipoprotein particle clearance
GO:0034447 very-low-density lipoprotein particle clearance
GO:0046911 metal chelating activity
GO:0042627 chylomicron
GO:0042632 cholesterol homeostasis
GO:0042803 protein homodimerization activity
GO:0043025 neuronal cell body
GO:0043407 negative regulation of MAP kinase activity
GO:0043537 negative regulation of blood vessel endothelial cell migration
GO:0043691 reverse cholesterol transport
GO:0045541 negative regulation of cholesterol biosynthetic process
GO:0046907 intracellular transport
GO:0046982 protein heterodimerization activity
GO:0048156 tau protein binding
GO:0048168 regulation of neuronal synaptic plasticity
GO:0050728 negative regulation of inflammatory response
GO:0070326 very-low-density lipoprotein particle receptor binding
GO:0050750 low-density lipoprotein particle receptor binding
GO:0051000 positive regulation of nitric-oxide synthase activity
GO:0051044 positive regulation of membrane protein ectodomain proteolysis
GO:0060228 phosphatidylcholine-sterol O-acyltransferase activator activity

MCA5639

0410R 151531

MCA5639GA

0410 156231

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