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Apolipoprotein B Antibody

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  • Goat anti Human Apolipoprotein B
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1267E, IP, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat anti Human Apolipoprotein B antibody detects human Apolipoprotein B (ApoB), a large secretory protein with a molecular weight of ~550 kDa that plays a central role in lipoprotein metabolism.

      ApoB exists in the plasma in 2 main isoforms, ApoB100 and ApoB48. ApoB100 is synthesised in the liver, and is the major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL), and is absolutely required for their formation.ApoB100 acts as a ligand for LDL receptors in various cells throughout the body, allowing cells to internalise LDL and absorb cholesterol.

      ApoB48 is expressed in the intestine and is present on chylomicrons and their remnants, and lacks the C-terminal LDL-receptor binding region of ApoB100. Elevated plasma concentrations of ApoB containing lipoproteins are key risk factors for the development of atherosclerosis.
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to Apolipoprotein B were raised by repeated immunisations of goats with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.01%Sodium Azide (NaN3)
    • Immunogen
      Apolipoprotein type B, isolated from human plasma by density gradient centrifugation followed by HPLC purification.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Borate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/2001/1000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1267 detects a band of approximately 550kDa.
    • Instructions For Use

    Additional Apolipoprotein B Antibody Formats

    Formats Applications Sizes available
    Apolipoprotein B Antibody : Purified E, IP, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Further Reading

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