APOBEC3G Antibody

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APOBEC3G Antibody gallery image 1

Western blot analysis of whole cell lysate from Caco-2 human colorectal carcinoma cell line probed with Rabbit anti Human APOBEC3G (AHP995)

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APOBEC3G Antibody gallery image 2

Immunohistochemical staining of human spleen with Rabbit anti Human AOPBEC3G (AHP995)

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  • Rabbit anti Human APOBEC3G (N-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP995P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP995
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Human AID antibody recognises an epitope within the N-terminal region (NT) of APOBEC3G (apolipoprotein B mRNA-editing, enzyme-catalytic, polypeptide-like 3G), a ~46 kDa multi-isoform endogenous deoxycytidine deaminase, which plays a major role in the defence of T lymphocytes against retroviral infection.

      The incorporation of APOBEC3G into HIV-1 virion DNA, results in the deamination of dC to dU in minus-strand viral DNA during reverse transcription, thereby generating G to A hypermutations in the newly-synthesised viral DNA. The effect of APOBEC3G is counteracted by the HIV protein Vif, thought to induce the degradation of APOBEC3G through a ubiquitin-dependant proteasomal pathway.

      Rabbit anti Human AID antibody (AHP995) also detects the APOBEC3F isoform (apolipoprotein B mRNA-editing, enzyme-catalytic, polypeptide-like 3F).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting5ug/ml
      Immunohistology - Paraffin(1)1ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Spleen
    • Immunofluorescence
    • Western Blotting
      AHP995 detects a band of approximately 46kDa in Human CaCO-2 cell lysates.
    • Instructions For Use

    Additional APOBEC3G Antibody Formats

    Formats Applications Sizes available
    APOBEC3G Antibody : Purified P *, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
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          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Spleen

              Further Reading

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