Serum Amyloid A (apo-SAA) is an acute phase response protein and injury specific apolipoprotein, synthesized primarily in the liver, which is found in trace amounts in association with high density lipoproteins (HDL) in human serum, but increases dramatically during an acute response to inflammation, infection, trauma, and malignant neoplasia, to become the most abundant HDL apolipoprotein. The acute phase isoforms of apo-SAA are encoded by the genes SAA1 (Apo-SAA1) and SAA2 (Apo-SAA2), and both include allelic variants.
Recombinant Apo-SAA corresponds to human apo-SAA1alpha, but lacks the N-terminal methionine, and contains a substitution of asparagine for aspartic acid at position 60 and arginine for histidine at position 71(the later two substituted residues are present in apo-SAA2beta).
Purified recombinant protein - lyophilised
Reconstitute with 0.5ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Purified recombinant apo-SAA expressed in E.coli
For the majority of in vitro applications, apo-SAA exerts its biological activity in the concentration range of 10-50 µg/ml.
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1mg/ml after reconstitution
Reagents In The Kit
Preparing The Antibody
5mM Tris, pH7.8
Prior to reconstitution store at +4oC. After reconstitution store at -20oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.