APH-1A Antibody

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Human Cerebral Cortex cell lysate probed with Goat anti Human APH-1A (AHP2250)

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  • Goat anti Human APH-1A
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2250E, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP2250
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Human APH-1A antibody recognises human APH-1A (gamma-secretase subunit APH-1A), otherwise known as anterior pharynx defective 1 homolog A, an integral transmembrane protein, and member of the APH-1 family, which is predominantly located in the endoplasmic reticulum and cis-Golgi, and is an essential component of the endoprotease gamma-secretase complex.

      The gamma-secretase complex, consisting of APH-1 (APH-1A or APH-1B), presenilin-1 or -2 (PSEN1 or PSEN2), nicastrin (NCSTN), and Pen-2 (PEN2), plays a vital role in the evolutionarily conserved Notch signaling pathway, by catalyzing the intramembrane S3 cleavage of ligand-activated Notch receptor proteins (Notch1-4), resulting in the generation and release of an activated NICD fragment, which then translocates to the nucleus and activates the transcription of downstream effector genes.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      MouseExpected from Sequence
      RatExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to human APH-1A was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      0.5% Bovine Serum Albumin
    • Immunogen
      Synthetic peptide sequence C-HVTDRSDARLQYG from the internal region of human APH-1A (NP_001071096.1; NP_057106.2).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      endoplasmic reticulum membrane
      positive regulation of catalytic activity
      protein binding
      Golgi cisterna membrane
      nerve growth factor receptor signaling pathway
      apoptosis
      Notch signaling pathway
      membrane protein intracellular domain proteolysis
      protein processing
      integral to plasma membrane
      membrane protein ectodomain proteolysis
      Notch receptor processing
      amyloid precursor protein catabolic process
      induction of apoptosis by extracellular signals
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/128,000
      Western Blotting0.050.1ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP2250 detects a band of approximately 26kDa in Human cerebral cortex cell lysates.
    • Instructions For Use

    Additional APH-1A Antibody Formats

    Formats Applications Sizes available
    APH-1A Antibody : Purified E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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