APEX1 Antibody (PrecisionAbTM Antibody) | CPTC15

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APEX1 Antibody (PrecisionAb<sup>TM</sup> Antibody) | CPTC15 gallery image 1

Western blot analysis of whole cell lysates probed with APEX1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 1 second exposure. Arrow points to APEX1 (molecular weight 36 kDa)

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  • APEX1 Antibody (PrecisionAb<sup>TM</sup> Antibody) | CPTC15 thumbnail image 1
  • APEX1 Antibody with Control Lysate
  • Mouse anti APEX1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    PrecisionAb™ Monoclonal
  • Isotype
    IgG1
  • Format
    Purified
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    VMA00561WBdatasheet pdfdatasheet pdf100 µl
    VMA00561
    VMA00561KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
    VMA00561KT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    References
    Reviews
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    • Mouse anti Human APEX1 antibody recognizes the apurinic/apyrimidinic endodeoxyribonuclease 1, also known as AP endonuclease class I, AP lyase or protein REF-1.

      Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. The APEX1 gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein (provided by RefSeq, Jul 2008).

      Mouse anti Human APEX1 antibody detects a band of 36 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
      STAR207P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
      STAR207P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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