Annexin V (ANXA5), otherwise known as Lipocortin V, is a calcium-dependent phospholipid-binding protein and member of the annexin family, which binds with high affinity and selectivity to phosphatidylserine (PS) and provides a reliable diagnostic tool for the indication of early programmed cell death (apoptosis). Annexin V has also been identified as an anticoagulant protein in the blood coagulation cascade, by acting as an inhibitor of prothrombin activation.
Purified recombinant protein - liquid
Purified recombinant 6xHis-tagged ANXA5 expressed in E.coli
Approx. Protein Concentrations
38.6kD (342 amino acid residues with tag)
Reagents In The Kit
Preparing The Antibody
20mM Tris HCl 0.5m Nacl 100mM Imidazole 1mM Beta-mercaptoethanol, pH8.0
Store at -70oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
His-tag is a registered trademark of EMD Biosciences.
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.