Annexin V

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  • Recombinant Human Annexin V
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP229E, WBdatasheet pdfdatasheet pdf25 µg
    PHP229
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Annexin V (ANXA5), otherwise known as Lipocortin V, is a calcium-dependent phospholipid-binding protein and member of the annexin family, which binds with high affinity and selectivity to phosphatidylserine (PS) and provides a reliable diagnostic tool for the indication of early programmed cell death (apoptosis). Annexin V has also been identified as an anticoagulant protein in the blood coagulation cascade, by acting as an inhibitor of prothrombin activation.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified recombinant protein - liquid
    • Reconstitution
    • Preparation
      Purified recombinant 6xHis-tagged ANXA5 expressed in E.coli
    • Preservative Stabilisers
      None present
    • Purity
    • Approx. Protein Concentrations
      0.1 mg/ml
    • Molecular Weight
      38.6kD (342 amino acid residues with tag)
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      20mM Tris HCl
      0.5m Nacl
      100mM Imidazole
      1mM Beta-mercaptoethanol, pH8.0
    • Storage
      Store at -70oC.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • GO Terms
      cytoplasm
      phospholipase inhibitor activity
      anti-apoptosis
      negative regulation of coagulation
      blood coagulation
      calcium ion binding
      signal transduction
      calcium-dependent phospholipid binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Annexin V Formats

    Formats Applications Sizes available
    Annexin V : Rec. Protein E, WB 25 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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