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Annexin V

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  • Recombinant Human Annexin V
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP186IAdatasheet pdfdatasheet pdf30 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Annexin V is a phospholipid binding protein which binds with high affinity and selectivity to phosphatidylserine (PS), in the presence of calcium. The translocation of PS from the cytosolic leaflet to the outer leaflet of the plasma membrane, due to a loss in plasma membrane asymmetry during apoptosis, acts as an in vivo signal for the removal of the dying cell by phagocytosis.

      Detection of PS using Annexin V provides a reliable diagnostic tool for the indication of early programmed cell death. Bio-Rad recommend the use of Annexin V kits for this purpose.

      Annexin V has also been identified as an inhibitor of prothrombin activation in the blood coagulation cascade, and the presence of antibodies to Annexin V are associated with systemic lupus erythematosus (SLE), recurrent spontaneous abortions and systemic sclerosis (SSc).
    • Intended Use
    • Target Species
    • Product Form
      Purified recombinant protein - lyophilised
    • Reconstitution
      Reconstitute with 1.0ml distilled water at 30oC for 5 minutes.
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Purified recombinant Annexin V expressed in E.coli.
    • Preservative Stabilisers
      None present
    • Purity
      >98% by SDS PAGE/HPLC analysis
    • Approx. Protein Concentrations
      Total protein concentration 30ug/ml after reconstitution
    • Molecular Weight
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      20mM Phosphate Buffer
      0.02% Tween 80
      130mM Arginine HCl.
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      6 months from date of reconstitution.
    • GO Terms
      negative regulation of coagulation
      calcium ion binding
      blood coagulation
      signal transduction
      calcium-dependent phospholipid binding
      phospholipase inhibitor activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      In vitro Assay

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Annexin V Formats

    Formats Applications Sizes available
    Annexin V : Rec. Protein IA 30 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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