Annexin V Antibody | VAA-33

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  • Mouse anti Human Annexin V:FITC
  • Mouse anti Human Annexin V:Biotin
  • Mouse anti Human Annexin V
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    VAA-33
  • Isotype
    IgG2a
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2712E, Fdatasheet pdfdatasheet pdf0.1 mg
    MCA2712
    MCA2712FFdatasheet pdfdatasheet pdf100 Tests
    MCA2712F
    MCA2712BFdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA2712B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Annexin V antibody, clone VAA-33 specifically recognizes human Annexin V, a phospholipid binding protein which binds with high affinity and selectivity to phosphatidylserine (PS), in the presence of calcium.

      The translocation of PS from the cytosolic leaflet to the outer leaflet of the plasma membrane, due to a loss in plasma membrane asymmetry during apoptosis, acts as an in vivo signal for the removal of the dying cell by phagocytosis. Detection of PS using Annexin V therefore provides a reliable diagnostic tool for the indication of early programmed cell death.

      Annexin V has also been identified as an anticoagulant protein in the blood coagulation cascade, by acting as an inhibitor of prothrombin activation. The presence of antibodies to Annexin V, are associated with systemic lupus erythematosus (SLE), recurrent spontaneous abortions and systemic sclerosis (SSc).

      Mouse anti Human Annexin V antibody, clone VAA-33 has been reported to work in Western blotting applications.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Biotin - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
      Purified IgG prepared by affinity chromatography on Protein G
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      0.02%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      0.02%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      0.09%Sodium Azide (NaN3)
    • Immunogen
      Recombinant Annexin V
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.2 mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
      TRIS buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      blood coagulation
      calcium ion binding
      signal transduction
      cytoplasm
      negative regulation of coagulation
      calcium-dependent phospholipid binding
      phospholipase inhibitor activity
      anti-apoptosis
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional Annexin V Antibody Formats

    Formats Clone Applications Sizes available
    Annexin V Antibody : Purified VAA-33 E, F 0.1 mg
    Annexin V Antibody : Biotin VAA-33 F 100 Tests/1ml
    Annexin V Antibody : FITC VAA-33 F 100 Tests
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
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      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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      STAR87A
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      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
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      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
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      Human anti Mouse IgG2a:FITCHCA037F0.1 mgF
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      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG2a:HRPHCA037P0.1 mgE
      HCA037P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
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      STAR13B
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      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative Control:FITCMCA929F100 TestsF
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Human Annexin V:BiotinMCA2712B100 Tests/1mlF
          MCA2712B
          Annexin V:Biotin Assay KitANNEX100B100 TestsF
          ANNEX100B
          Annexin V:Biotin Assay KitANNEX300B300 TestsF
          ANNEX300B
          Annexin V:FITC Assay KitANNEX100F100 TestsF
          ANNEX100F
          Annexin V:FITC Assay KitANNEX300F300 TestsF
          ANNEX300F
          Mouse anti Human Annexin VMCA27120.1 mgE, F
          MCA2712
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Annexin V:Biotin Assay KitANNEX100B100 TestsF
          ANNEX100B
          Annexin V:Biotin Assay KitANNEX300B300 TestsF
          ANNEX300B
          Annexin V:FITC Assay KitANNEX100F100 TestsF
          ANNEX100F
          Mouse anti Human Annexin V:FITCMCA2712F100 TestsF
          MCA2712F
          Annexin V:FITC Assay KitANNEX300F300 TestsF
          ANNEX300F
          Mouse anti Human Annexin VMCA27120.1 mgE, F
          MCA2712
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Human Annexin V:BiotinMCA2712B100 Tests/1mlF
          MCA2712B
          Annexin V:Biotin Assay KitANNEX100B100 TestsF
          ANNEX100B
          Annexin V:Biotin Assay KitANNEX300B300 TestsF
          ANNEX300B
          Annexin V:FITC Assay KitANNEX100F100 TestsF
          ANNEX100F
          Mouse anti Human Annexin V:FITCMCA2712F100 TestsF
          MCA2712F
          Annexin V:FITC Assay KitANNEX300F300 TestsF
          ANNEX300F

          Recommended Positive Controls

            Histology Controls

              Further Reading

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