Annexin II Antibody

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  • Goat anti Human Annexin II (N-Terminal)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    OBT1388E, WBdatasheet pdfdatasheet pdf0.1 mg
    OBT1388
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Human Annexin II (N-Terminal) antibodyrecognizes an epitope within the N terminal (NT) region of Annexin II, a member of a family of structurally related calcium binding proteins implicated in mediating the the intracellular action of calcium. Cellular levels of annexin II mRNA and protein are strictly regulated during the mammalian cell cycle with maximal expression as cells enter S-phase.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      RatExpected from Sequence
      Mouseyes
      BovineExpected from Sequence
      PigExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to human Annexin II was raised by repeated immunisation of goats with highly purified antigen. Purified IgG prepared by affinity chromatography
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      0.5% Bovine Serum Albumin
    • Immunogen
      Peptide sequence STVHEILCKLSLEGD corresponding to the N-terminal region of Annexin II (NP_001002858.1; NP_004030.1; NP_001002857.1).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      positive regulation of vesicle fusion
      phospholipase inhibitor activity
      basement membrane
      calcium ion binding
      cytoskeletal protein binding
      melanosome
      soluble fraction
      calcium-dependent phospholipid binding
      skeletal system development
      midbody
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/4000
      Western Blotting1 ug/ml3 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      OBT1388 detects a band of approximately 35 kDa in A431 and A549 cell lysates
    • Instructions For Use

    Additional Annexin II Antibody Formats

    Formats Applications Sizes available
    Annexin II Antibody : Purified E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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