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Annexin A1 antibody | CPTC22

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KO Validated
IP-MS Validated
Anti Annexin A1 Antibody, clone CPTC22 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti Annexin A1 Antibody, clone CPTC22 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti Annexin A1 Antibody, clone CPTC22 (PrecisionAb Monoclonal Antibody) thumbnail image 3 Anti Annexin A1 Antibody, clone CPTC22 (PrecisionAb Monoclonal Antibody) thumbnail image 4 Anti Annexin A1 Antibody, clone CPTC22 (PrecisionAb Monoclonal Antibody) thumbnail image 5
Anti Annexin A1 Antibody, clone CPTC22 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with annexin A1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 1 second exposure. Arrow points to annexin A1 (molecular weight 39 kDa)
Anti Annexin A1 Antibody, clone CPTC22 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of Annexin A1 knockout HeLa and wild type HeLa (WT) whole cell lysates probed with (A) Mouse anti Annexin A1 antibody (VMA00558) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 25 second and (B) 60 second exposure.
Anti Annexin A1 Antibody, clone CPTC22 (PrecisionAb Monoclonal Antibody) gallery image 3
Normalized signal intensity of Annexin A1 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.
Anti Annexin A1 Antibody, clone CPTC22 (PrecisionAb Monoclonal Antibody) gallery image 4
Immunoprecipitation of Annexin A1 from K562 whole cell lysate. The Input lane is 10% input; the ANNEXIN A1-IP lane is the immunoprecipitation with the Annexin A1 antibody (2.5 µg, VMA00558); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 µg, MCA929). Western blot analysis was performed using Annexin A1 antibody (1/1,000, VMA00558) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to Annexin A1.
Anti Annexin A1 Antibody, clone CPTC22 (PrecisionAb Monoclonal Antibody) gallery image 5
HeLa cells stained with Mouse anti Annexin A1 antibody (VMA00558) at a 1/500 dilution followed by detection with an Alexa Fluor 488 conjugated Goat anti Mouse IgG (H/L): antibody at a 1/800 dilution (green) and PureBlu™ DAPI (1351303) (blue) as a nuclear counterstain.

Mouse anti Annexin A1

Product Type
PrecisionAb Monoclonal
Clone
CPTC22
Isotype
IgG2a
Format
Purified
Specificity
Annexin A1

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Product Code Applications Pack Size List Price Your Price Qty
VMA00558
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IF IP WB 100 µl loader
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loader

Mouse anti Human annexin A1 antibody recognizes annexin A1, also known as ANXA1 or calpactin II.

This ANXA1 gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors (provided by RefSeq, Dec 2014)

Mouse anti Human annexin A1 antibody detects a band of 39 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti annexin A1 detects a band of approximately 39 kDa in K562 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Recombinant protein of human annexin A1
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P04083
Entrez Gene
ANXA1
GO Terms
GO:0005509 calcium ion binding
GO:0001533 cornified envelope
GO:0030674 protein binding, bridging
GO:0005102 receptor binding
GO:0005576 extracellular region
GO:0005198 structural molecule activity
GO:0005544 calcium-dependent phospholipid binding
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005929 cilium
GO:0006629 lipid metabolic process
GO:0006916 anti-apoptosis
GO:0006928 cellular component movement
GO:0006954 inflammatory response
GO:0007166 cell surface receptor linked signaling pathway
GO:0016323 basolateral plasma membrane
GO:0018149 peptide cross-linking
GO:0019834 phospholipase A2 inhibitor activity
GO:0030216 keratinocyte differentiation
GO:0031340 positive regulation of vesicle fusion
GO:0046632 alpha-beta T cell differentiation

VMA00558

170420

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