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Amyloid Precursor Protein Antibody

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  • Rabbit anti Human Amyloid Precursor Protein (KPI Domain)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP665IP*, P *, WB*datasheet pdfdatasheet pdf0.1 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti Human Amyloid Precursor Protein antibody recognizes an epitope in the Kunitz protease inhibitor (KPI) domain of human amyloid precursor protein (APP).

      Only the APP-751 and APP-770 isoforms contain a KPI domain, it being absent from the APP-695 isoform that is most abundant in neuronal tissue. However, there is evidence that suggests KPI containing isoforms may be important in the pathogenesis of Alzheimer disease.

      Rabbit anti Human Amyloid Precursor Protein antibody recognizes a band of ~115kDa in NTera2/DI cell lysates using western blotting under reducing conditions.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Serum - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to human APP were raised by repeated immunisations of rabbits with highly purified antigen.
    • Preservative Stabilisers
      0.02%Sodium Azide
    • Immunogen
      Synthetic peptide composed of amino acids 292-312 from the KPI domain of human APP.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin(1)1/400
      Immunoprecipitation(2)Neat, 5ul/ml
      Western Blotting(3)1/5001/1000
      This product requires antigen retrieval using heat treatment prior to staining of paraffin embedded sections. 0.05M Tris pH7.0, followed by reduction and alkylation is recommended (Campbell et al. 1999).
      This product requires sample reduction and alkylation prior to immunoprecipitation.
      Antigen detection may be enhanced following reduction and alkylation of the sample prior to blocking and antibody incubation.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Alzheimer's disease brain.
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Amyloid Precursor Protein Antibody Formats

    Formats Applications Sizes available
    Amyloid Precursor Protein Antibody : Serum IP*, P *, WB* 0.1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Alzheimer's disease brain.


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