AMPK Alpha 2 Antibody

AMPK Alpha 2 Antibody gallery image 1

Western blotting analysis of human liver lysate (35µg protein in RIPA buffer) probed with Goat anti Human AMPK alpha antibody (AHP2256) at 1.0μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence

AMPK Alpha 2 Antibody gallery image 2

Western blotting analysis of mouse (A) and rat (B) heart lysate (35μg protein in RIPA buffer) probed with Goat anti HUman AMPK alpha antibody (AHP2256) at 0.3μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence

AMPK Alpha 2 Antibody gallery image 3

ELISA assay for the evaluation of AMPK alpha levels using Goat anti Human AMPK alpha antibody (AHP2256) as the detection reagent at 1.5μgml-1 with a rabbit polyclonal anti AMPK alpha as the capture reagent

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  • Goat anti Human AMPK Alpha 2
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2256E, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat anti Human AMPK alpha 2 antibody recognizes human5′-AMP-activated protein kinase catalytic subunit alpha-2, also known as AMPK alpha 2, ACACA kinase, Acetyl-CoA carboxylase kinase or Hydroxymethylglutaryl-CoA reductase kinase, encoded by the PRKAA2 gene. AMPK alpha 2 is a a Serine/threonine-protein kinase and the catalytic subunit of the AMP-activated protein kinase (AMPK), a central metabolic sensor, essential for the regulation of fatty acid and cholesterol synthesis, in response to fluctuations in nutrient and intracellular energy levels.

      AMPK has been implicated as a suppressor of cell proliferation of both normal and malignant cells. AMPK binding to the PS domain of ULK1, is essential for ULK1-mediated autophagy (Roach et al. 2011), and that the phosphorylation of raptor by AMPK, plays an important role in autophagy induction (Egan et al. 2011).
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      DogExpected from Sequence
      BovineExpected from Sequence
      PigExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to human AMPK alpha 2 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      0.5% Bovine Serum Albumin
    • Immunogen
      Synthetic peptide sequence CPLDALNTTKP from the internal region of AMPK alpha 2 (NP_006243.2).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      insulin receptor signaling pathway
      protein phosphorylation
      energy reserve metabolic process
      fatty acid biosynthetic process
      regulation of fatty acid biosynthetic process
      cholesterol biosynthetic process
      ATP binding
      cell cycle arrest
      regulation of fatty acid oxidation
      carnitine shuttle
      metal ion binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1.03.0 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP2256 detects a band of approximately 65kDa in Human heart and liver cell lysates.
    • Instructions For Use

    Additional AMPK Alpha 2 Antibody Formats

    Formats Applications Sizes available
    AMPK Alpha 2 Antibody : Purified E, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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