AMPK Alpha 1 Antibody (PrecisionAbTM Antibody)

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AMPK Alpha 1 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with AMPK alpha 1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 157 second exposure. Arrow points to AMPK alpha 1 (molecular weight 64 kDa)

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  • AMPK Alpha 1 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Mouse anti AMPK Alpha 1
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    AMPK alpha 1 antibody recognizes 5'-amp-activated protein kinase catalytic subunit alpha-1, also known as 5'-AMP-activated protein kinase, catalytic alpha-1 chain, ACACA kinase, AMP-activated protein kinase, catalytic, alpha-1, AMPK alpha 1, HMGCR kinase, acetyl-CoA carboxylase kinase, hydroxymethylglutaryl-CoA reductase kinase or tau-protein kinase PRKAA1. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00246WBdatasheet pdfdatasheet pdf100 µl
      VMA00246
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human AMPK alpha 1 antibody recognizes the 5'-AMP-activated protein kinase catalytic subunit alpha-1, also known as 5'-AMP-activated protein kinase catalytic subunit alpha-1, 5'-AMP-activated protein kinase, catalytic alpha-1 chain, ACACA kinase, AMP -activate kinase alpha 1 subunit, AMP-activated protein kinase, catalytic, alpha-1, AMPK alpha 1, AMPK subunit alpha-1, HMGCR kinase, acetyl-CoA carboxylase kinase, hydroxymethylglutaryl-CoA reductase kinase and tau-protein kinase PRKAA1.

        Encoded by PRKAA1 gene, AMPK alpha 1 belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed (provided by RefSeq, Jul 2008).

        Mouse anti Human AMPK alpha 1 antibody detects a band of 64 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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