Amphiregulin Antibody

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Staining of formalin fixed, paraffin embedded human adrenal gland with Rabbit anti Human amphiregulin (AHP2046)

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  • Rabbit anti Human Amphiregulin
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2046IP, Pdatasheet pdfdatasheet pdf50 µg
    AHP2046
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Human Amphiregulin antibody recognizes human amphiregulin, also known as colorectum cell derived growth factor or CRDGF> Amphiregulin is a 252 amino acid ~28 kDa single pass transmembrane glycoprotein and ligand for the EGF receptor (Plowman et al 1990) as a mature 84 amino acid protein.

      Amphirequlin has also been identified as homologous to shwannoma - derived growth factor, identified from a shwannoma derived cell line JS1 (Kimura et al. 1990).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      MouseExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to human Amphiregulin was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.05% Sodium Azide (NaN3)
      0.2% Bovine Serum Albumin
    • Immunogen
      Synthetic peptide sequence corresponding to amino acids 8-26 of Human amphiregulin.
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 0.2mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      cell surface
      cell-cell signaling
      epidermal growth factor receptor signaling pathway
      positive regulation of DNA replication
      integral to membrane
      growth factor activity
      G-protein coupled receptor protein signaling pathway
      cell proliferation
      cytokine activity
      extracellular space
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin10ug/ml
      Immunoprecipitation

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
      This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
    • Histology Positive Control Tissue
      Human adrenal gland
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Amphiregulin Antibody Formats

    Formats Applications Sizes available
    Amphiregulin Antibody : Purified IP, P 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Histar Detection SystemSTAR3000B150 TestsC, P
          STAR3000B
          Histar Detection SystemSTAR3000A50 TestsC, P
          STAR3000A
          Histar Detection SystemSTAR3000C500 TestsC, P
          STAR3000C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Human adrenal gland

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