Alpha Fetoprotein Antibody (PrecisionAbTM Antibody)

//www.bio-rad-antibodies.com/human-alpha-fetoprotein-antibody-vma00036.html?RenderPageSpecific=1
Alpha Fetoprotein Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with alpha fetoprotein antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/50,000, STAR207P) and visualized on the ChemiDoc MP with 300 second exposure, Arrow points to alpha fetoprotein (molecular weight 69 kDa)

Enlarge
  • Alpha Fetoprotein Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Alpha Fetoprotein Antibody with Control Lysate
  • Mouse anti Alpha Fetoprotein
  • Alpha Fetoprotein Antibody with Control Lysate
(Rated 0.0 out of 5 based on 0 customer reviews)
    Alpha fetoprotein antibody recognizes alpha fetoprotein, also known as alpha-1-fetoprotein or alpha-fetoglobulin. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00036WBdatasheet pdfdatasheet pdf100 µl
      VMA00036
      VMA00036KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VMA00036K
      VMA00036KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00036KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Mouse anti Human alpha fetoprotein antibody recognizes alpha fetoprotein (AFP), a 609 amino acid member of the ALB/AFP/VDB family containing 3 albumin domains. Human alpha fetoprotein, also known as alpha fetoglobulin is a plasma protein found in fetuses over four weeks old, produced by the yolk sac and the fetal liver, with the highest concentration reached during the 12th to 16th week of gestation. It binds and transports various ligands such as bilirubin, fatty acids, steroids, heavy metals and various drugs (Aoyagi et al. 1979). After birth, plasma levels drop to trace amounts in healthy adults.

        Elevated AFP levels are indicative of certain cancers, particularly hepatocellular carcinoma, cancer of the testes or ovaries or a malignant teratoma. During pregnancy increased levels of AFP may indicate neural tube defects such as spina bifida (Bredaki et al. 2012), while decreased levels could indicate Down’s syndrome (Norgaard-Pedersen et al. 1976).

        Mouse Anti Human Alpha Fetoprotein antibody recognizes alpha fetoprotein as a band of ~69 kDa in HepG2 whole cell lysate by Western blotting under reducing conditions and does not cross-react with human albumin.
      • Target Species
        Human
      • Western Blotting
        Anti alpha fetoprotein antibody detects a band of approximately 69 kDa in HepG2 cell lysates.
        Anti alpha fetoprotein antibody detects a band of approximately 69 kDa in HepG2 cell lysates.
        Anti alpha fetoprotein antibody detects a band of approximately 69 kDa in HepG2 cell lysates.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
        Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Purified human alpha fetoprotein
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HepG2 lysate lyophilized in RIPA buffer.
        400μg HepG2 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

                Write your review

                Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

                Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

                Contact Information


                Online Payment Methods:

                1. Invoice / Purchase Order
                2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
                credit cards accepted