Alpha Fetoprotein Antibody

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Alpha Fetoprotein Antibody gallery image 1

Published customer image:
Rabbit anti Human alpha Fetoprotein antibody used for the detection of alpha fetoprotein in dendritic cells by immunofluorescence.
Image caption:
Adenovirally-expressed AFP, but not eGFP-AFP, is secreted by transduced DC. a DC were transduced with AdVhAFP (left panel), AdVeGFP-AFP (middle panel), or AdVTyrosinase at MOI 2000 for 3 hr. After 48 hr, cells were fixed and stained for AFP (left and middle panels), actin (left and right panels), or Tyrosinase (right panel). Antibody staining of AdVeGFP-AFP-transduced cells with an anti-AFP antibody reveals that AFP and GFP colocalize, as expected. Representative images from three independent experiments are shown. Images were taken using a 63x objective. b DC from healthy donors (n?=?3) were transduced as above and cultured in DC media for 24, 48, or 72 hr. Supernatants were analyzed by AFP ELISA. Data are reported as the mean?±?SD

From: Pardee AD, Yano H, Weinstein AM, Ponce AA, Ethridge AD, Normolle DP, Vujanovic L, Mizejewski GJ, Watkins SC, Butterfield LH. Route of antigen delivery impacts the immunostimulatory activity of dendritic cell-based vaccines for hepatocellular carcinoma.
J Immunother Cancer. 2015 Jul 21;3:32.

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  • Rabbit anti Human Alpha Fetoprotein
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP457C, Pdatasheet pdfdatasheet pdf1 ml
    AHP457
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Human alpha Fetoprotein antibody recognizes human alpha fetoprotein, and shows positive staining of parenchymal cells of fetal liver and neoplastic liver cells showing yolk sac differentiation in germ cell tumours.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Pigyes
      Sheepyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to human AFP were raised by repeated immunisation of rabbits with highly purified antigen
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Human Alpha-1-Fetoprotein (cord serum)
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1/101/40
      Immunohistology - Paraffin1/101/40

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
      This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
    • Histology Positive Control Tissue
      Hepalocellular carcinoma, fetal liver, germ cell tumors
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Alpha Fetoprotein Antibody Formats

    Formats Applications Sizes available
    Alpha Fetoprotein Antibody : Concentrate C, P 1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Hepalocellular carcinoma, fetal liver, germ cell tumors

              References

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