Alpha Fetoprotein Antibody | 125.7A2

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Human alpha fetoprotein (PHP152) detected in a sandwich ELISA using Mouse anti Human alpha fetoprotein (MCA5862G) as the capture reagent and Mouse anti Human alpha fetoprotein:HRP (MCA5861P) as the detection reagent

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  • Alpha Fetoprotein Antibody | 125.7A2 thumbnail image 1
  • Mouse anti Human Alpha Fetoprotein
  • Mouse anti Human Alpha Fetoprotein:HRP
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  • Product Type
    Monoclonal Antibody
  • Clone
    125.7A2
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5861PEdatasheet pdfdatasheet pdf0.1 mg
    MCA5861P
    MCA5861GEdatasheet pdfdatasheet pdf1 mg
    MCA5861G
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human alpha fetoprotein (clone 125.7A2) is a monoclonal antibody recognising alpha fetoprotein (AFP), a member of the albuminoid protein superfamily, along with albumin and vitamin D binding protein. Members of the albuminoid superfamily comprise molecules with three characteristic globular domains (Mizejewski 1995).

      AFP is a ~70kD plasma protein found in fetuses over four weeks old, produced by the yolk sac and the fetal liver, with the highest concentration reached during the 12th to 16th week of gestation. AFP acts as a transport protein for various ligands such as bilirubin, fatty acids, steroids and heavy metals. After birth, plasma AFP levels drop rapidly with only trace amounts remaining in healthy adults. Changes in plasma levels of AFP have been documented in a variety of cancers including hepatocellular cancer (Sato et al. 1993).

      Clone 125.7A2 shows <0.1% reactivity with human serum albumin and IgG. Strong expression of AFP has been noted in the hepatic cell line HepG2.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.01% Thiomersal
      HRP Stabiliser (BUF052A)
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
      18 months from date of despatch
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA5µg/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA5µg/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
      This product may be used as a detection reagent in a sandwich ELISA together with MCA5862G as the capture reagent. PHP152 may be used as a standard.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Alpha Fetoprotein Antibody Formats

    Formats Clone Applications Sizes available
    Alpha Fetoprotein Antibody : Purified 125.7A2 E 1 mg
    Alpha Fetoprotein Antibody : HRP 125.7A2 E 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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