Alpha 2 Macroglobulin Antibody (PrecisionAbTM Antibody)

//www.bio-rad-antibodies.com/human-alpha-2-macroglobulin-antibody-vpa00200.html
Alpha 2 Macroglobulin Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with alpha 2 macroglobulin antibody followed by detection with HRP conjugated Rabbit anti Sheep IgG (H/L) (1/10,000, 5184-2504) and visualized on the ChemiDoc™ MP with 27 second exposure. Arrow points to alpha 2 macroglobulin (molecular weight 180 kDa)

Enlarge
  • Alpha 2 Macroglobulin Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Alpha 2 Macroglobulin Antibody with Control Lysate
  • Sheep anti Alpha 2 Macroglobulin
  • Alpha 2 Macroglobulin Antibody with Control Lysate
(Rated 0.0 out of 5 based on 0 customer reviews)
    alpha-2-Macroglobulin antibody recognizes alpha-2-macroglobulin, also known as C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 or alpha-2-M. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00200WBdatasheet pdfdatasheet pdf100 µl
      VPA00200
      VPA00200KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00200K
      VPA00200KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00200KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Sheep anti Human alpha 2 macroglobulin antibody recognizes the alpha 2 macroglobulin, also known as C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 and alpha-2-M.

        Alpha 2 macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. Alpha 2 macroglobulin is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits (provided by RefSeq, Jul 2008).

        Sheep anti Human alpha 2 macroglobulin antibody detects a band of 180 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti alpha 2 macroglobulin detects a band of approximately 180 kDa in HepG2 cell lysates.
        Anti alpha 2 macroglobulin detects a band of approximately 180 kDa in HepG2 cell lysates.
        Anti alpha 2 macroglobulin detects a band of approximately 180 kDa in HepG2 cell lysates.
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Sheep polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Sheep polyclonal antibody purified by affinity chromatography
        Sheep polyclonal antibody purified by affinity chromatography on Protein G
        Pack Size: 10 Westerns100μl Sheep polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Sheep polyclonal antibody purified by affinity chromatography
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Purified human alpha 2 macroglobulin
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HepG2 lysate lyophilized in RIPA buffer.
        400μg HepG2 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        regulation of small GTPase mediated signal transduction
        extracellular space
        platelet activation
        interleukin-1 binding
        blood coagulation, intrinsic pathway
        enzyme binding
        platelet alpha granule lumen
        GTPase activator activity
        cytosol
        negative regulation of complement activation, lectin pathway
        interleukin-8 binding
        small GTPase mediated signal transduction
        platelet degranulation
        tumor necrosis factor binding
        serine-type endopeptidase inhibitor activity
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rabbit anti Sheep IgG (H/L):HRP5184-25042 mgC, E, WB
        5184-2504
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rabbit anti Sheep IgG (H/L):HRP5184-25042 mgC, E, WB
        5184-2504
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rabbit anti Sheep IgG (H/L):HRP5184-25042 mgC, E, WB
        5184-2504

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

                Write your review

                Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

                Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

                Contact Information


                Online Payment Methods:

                1. Invoice / Purchase Order
                2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
                credit cards accepted