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Alpha 1 Microglobulin Antibody

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Native Human Alpha-1 Microglobulin detected with Sheep anti Human Alpha-1 Microglobulin:HRP (6220-0004P)

  • Alpha 1 Microglobulin Antibody thumbnail image 1
  • Sheep anti Human Alpha 1 Microglobulin
  • Sheep anti Human Alpha 1 Microglobulin:HRP
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    6220-0004GE, IDdatasheet pdfdatasheet pdf1 ml
    6220-0004PC, Edatasheet pdfdatasheet pdf1 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Sheep anti Human alpha 1 microglobulin antibody recognizes human alpha 1 microglobulin. Sheep anti Human alpha 1 microglobulin antibody does not demonstrate any cross reactivity with urine albumin, retinol binding protein or beta-2 microglobulin in performed testing.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
      Purified IgG prepared by affinity chromatography on Protein G
    • Antiserum Preparation
      Antisera to human alpha-1-microglubulin were raised by repeated immunisations of sheep with highly purified antigen.
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)>
      0.01% Thiomersal
      HRP Stabiliser (BUF052A)
    • Immunogen
      Highly pure human alpha-1-microglobulin, prepared from the urine of patients with chronic renal proteinuria (molecular weight 25-30 kD).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      plasma membrane
      cell adhesion
      heme catabolic process
      protein-chromophore linkage
      negative regulation of JNK cascade
      extracellular region
      protein homodimerization activity
      female pregnancy
      calcium oxalate binding
      transporter activity
      heme binding
      negative regulation of immune response
      calcium channel inhibitor activity
      serine-type endopeptidase inhibitor activity
      IgA binding
      interspecies interaction between organisms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1/101/500

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
      Excessive excretion of this protein has been known to indicate abnormalities of the renal tubules.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Alpha 1 Microglobulin Antibody Formats

    Formats Applications Sizes available
    Alpha 1 Microglobulin Antibody : HRP C, E 1 ml
    Alpha 1 Microglobulin Antibody : Purified E, ID 1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Sheep IgG (H/L):Biotin5184-23041.5 mlC, E, F, WB
      Donkey anti Sheep/Goat IgG:DyLight®488STAR88D488GA0.1 mgF, IF
      Donkey anti Sheep/Goat IgG:DyLight®549STAR88D549GA0.1 mgF, IF
      Donkey anti Sheep/Goat IgG:DyLight®649STAR88D649GA0.1 mgF, IF
      Donkey anti Sheep/Goat IgG:FITCSTAR88F1 mgC
      Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TMB Signal+BUF054A100 mlE
          TMB CoreBUF056A100 mlE
          TMB Core PrestainedBUF057A100 mlE
          TMB Core+BUF062A100 mlE

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • Alpha 1 Microglobulin Antibody thumbnail image 1

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