Alkaline Phosphatase

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  • Native Human Alkaline Phosphatase
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Purified Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    0300-2304Edatasheet pdfdatasheet pdf100 Units
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Native Human alkaline phosphatase is a liquid preparation of purified alkaline phosphatase derived from human placenta. Alkaline phosphatases are ubiquitous enzymes, occuring an species from bacteria to higher vertebrates, including man. They catalyse the hydrolysis of phosphate monoesters at basic pH values. In man there are four forms of alkaline phosphatase depending on tissue expression patterns. The four types are 'intestinal ALP', 'Placental ALP', 'germ cell ALP' and 'tissue non-specific ALP', also known as liver/ bone/ kidney ALP.
    • Intended Use
    • Target Species
    • Product Form
      Purified AP from human placenta - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      None present.
    • Activity
      1069U/mg solid

      One unit liberates one micromole of p-nitrophenol per minute at 37ºC, pH 10.15 in Tris buffer
    • Purity
      Biuret: 95%
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      cell surface
      integral to membrane
      anchored to membrane
      plasma membrane
      alkaline phosphatase activity
      metal ion binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Alkaline Phosphatase Formats

    Formats Applications Sizes available
    Alkaline Phosphatase : Purified E 100 Units
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls


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