ALK1 Antibody (PrecisionAbTM Antibody)

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ALK1 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with ALK1 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 7 second exposure. Arrow points to ALK1 (molecular weight 56 kDa)

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  • ALK1 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Rabbit anti ALK1
  • ALK1 Antibody with Control Lysate
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  • Product Type
    PrecisionAb™ Polyclonal
  • Isotype
    Polyclonal IgG
  • Format
    Purified
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    VPA00702WBdatasheet pdfdatasheet pdf100 µl
    VPA00702
    VPA00702KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
    VPA00702KT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    References
    Reviews
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    • Rabbit anti Human ALK1 antibody recognizes serine/threonine-protein kinase receptor R3, also known as ACVRLK1.

      The ACVRL1 gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein, sometimes termed ALK1, shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in ACVRL1 are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2 (provided by RefSeq, Jul 2008).

      Rabbit anti Human ALK1 antibody detects a band of 56 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Target Species
      Human
    • Western Blotting
      Rabbit anti ALK1 detects a band of approximately 56 kDa in K562 cell lysates
      Rabbit anti ALK1 detects a band of approximately 56 kDa in K562 cell lysates
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Preparation
      Rabbit polyclonal antibody purified by affinity chromatography
      20μl Rabbit polyclonal antibody purified by affinity chromatography
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Preservative Stabilisers
      0.01% Thimerosal
      1% Bovine Serum Albumin
      <50% Glycerol
      0.01% Thimerosal
      1% Bovine Serum Albumin
      <50% Glycerol
    • Immunogen
      Recombinant protein encompassing part of the C-terminal region of human ALK1
    • Instructions for Use
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
    • Lysate Composition
      400μg K562 lysate lyophilized in RIPA buffer.
    • Lysate Reconstitution
      - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
      - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
      Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
    • Storage
      Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

      Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
    • Shelf Life
      As supplied, 12 months from date of despatch.
      As supplied, 12 months from date of despatch
    • Acknowledgements
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      PrecisionAb™ is a trademark of Bio-Rad Laboratories
    • Regulatory
      For research purposes only
    • GO Terms
      activin receptor activity, type I
      ATP binding
      regulation of blood pressure
      SMAD binding
      metal ion binding
      negative regulation of cell growth
      angiogenesis
      transforming growth factor beta receptor signaling pathway
      positive regulation of transcription
      transforming growth factor beta binding
      cell surface
      wound healing, spreading of epidermal cells
      activin binding
      negative regulation of endothelial cell migration
      negative regulation of focal adhesion assembly
      integral to plasma membrane
      positive regulation of BMP signaling pathway
      protein phosphorylation
      receptor signaling protein serine/threonine kinase activity
      negative regulation of cell proliferation
    • UniProt
    • Entrez Gene
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
      STAR208P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
      STAR208P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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