AKT2 Antibody (PrecisionAbTM Antibody)

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AKT2 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with AKT2 antibody followed by detection with HRP conjugated Goat anti Rat IgG (1/10,000, STAR72) and visualized on the ChemiDoc MP with 60 second exposure. Arrow points to AKT2 (molecular weight 56 kDa)

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  • AKT2 Antibody with Control Lysate
  • Rat anti AKT2

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    Rat anti human AKT2 antibody recognizes v-Akt murine thymoma viral oncogene homolog 2, also known as protein kinase B beta, PKB beta, RAC-PK-Beta or protein kinase AKT-2. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG2a
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00091WBdatasheet pdfdatasheet pdf100 µl
      VMA00091
      VMA00091KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00091KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Rat anti Human AKT2 antibody recognizes AKT2, also known as protein kinase B beta, PKB beta and RAC-PK-beta.

        AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phosphorylating several known proteins (provided by RefSeq, Jul 2008).

        Rat anti Human AKT2 antibody detects a band of 56 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti AKT2 detects a band of approximately 56 kDa in HEK293 cell lysates.
        Anti AKT2 detects a band of approximately 56 kDa in HEK293 cell lysates.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        20μl Rat monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
        Rat monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Synthetic peptide corresponding to internal residues of human AKT2 protein
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HEK293 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        GO:0008286
        insulin receptor signaling pathway
        Related reagents
        GO:0046326
        positive regulation of glucose import
        Related reagents
        GO:0005515
        protein binding
        Related reagents
        GO:0004674
        protein serine/threonine kinase activity
        Related reagents
        GO:0005634
        nucleus
        Related reagents
        GO:0032000
        positive regulation of fatty acid beta-oxidation
        Related reagents
        GO:0005524
        ATP binding
        Related reagents
        GO:0005829
        cytosol
        Related reagents
        GO:0045725
        positive regulation of glycogen biosynthetic process
        Related reagents
        GO:0010748
        negative regulation of plasma membrane long-chain fatty acid transport
        Related reagents
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
        STAR72
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
        STAR72

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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