AKT2 Antibody (PrecisionAb^TM Antibody)

Anti AKT2 detects a band of approximately 56 kDa in HEK293 cell lysates.

Anti AKT2 detects a band of approximately 56 kDa in HEK293 cell lysates.

Purified IgG - liquid

Purified IgG - liquid

20μl Rat monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant

Rat monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant

Phosphate buffered saline

Phosphate buffered saline

0.09% Sodium Azide (NaN₃)

0.09% Sodium Azide (NaN₃)

Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

400μg HEK293 lysate lyophilized in RIPA buffer.

- If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.

Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

As supplied, 12 months from date of despatch.

As supplied, 12 months from date of despatch.

PrecisionAb™ is a trademark of Bio-Rad Laboratories.

PrecisionAb™ is a trademark of Bio-Rad Laboratories.