100% secure
Email this product page
Print this page

AKT2 Antibody | 1B3

AKT2 Antibody | 1B3 gallery image 1

Western blot analysis of AKT2 expression in Jurkat using Mouse anti Human AKT2:Azide Free (MCA2920Z)

AKT2 Antibody | 1B3 gallery image 2

Immunofluorescence showing AKT2 in HeLa cells using Mouse anti Human AKT2:Azide Free (MCA2920Z)

AKT2 Antibody | 1B3 gallery image 3

Sandwich ELISA analysis of human AKT2 binding using Mouse anti Human AKT2 antibody, clone 1F3 (MCA5507Z) as a capture reagent and biotinylated Mouse anti Human AKT2 antibody, clone 1B3 (MCA2920Z) as a detection reagent with purified Human AKT2 as antigen to generate the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). A neat serum sample (green) and plasma sample (orange) are indicated

  • AKT2 Antibody | 1B3 thumbnail image 1
  • AKT2 Antibody | 1B3 thumbnail image 2
  • AKT2 Antibody | 1B3 thumbnail image 3
  • Mouse anti Human AKT2:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2920ZIF, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Human AKT antibody, clone 1B3 recognizes human RAC-beta serine/threonine-protein kinase, also known as AKT2, RAC-PK-beta. Protein kinase Akt-2 or PKB beta. AKT2 is a 481 amino acid ~60kDa protein kinase containing a single AGC-kinase C-terminal domain, a PH domain and a protein kinase domain (Uniprot: P31751).

      Mouse anti Human AKT2 antibody, clone 1B3 has been successfully used as a detection reagent in the development of a sensitive sandwich ELISA in conjunction with Mouse anti AKT2 antibody, clone 1F3 as a capture reagent for the evaluation of AKT2 expression in human samples.
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 100 - 189 of human AKT2 with GST tag.
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      protein serine/threonine kinase activity
      ATP binding
      positive regulation of glucose import
      insulin receptor signaling pathway
      protein binding
      negative regulation of plasma membrane long-chain fatty acid transport
      positive regulation of glycogen biosynthetic process
      positive regulation of fatty acid beta-oxidation
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence0.110 ug/ml
      Western Blotting0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional AKT2 Antibody Formats

    Formats Clone Applications Sizes available
    AKT2 Antibody : Preservative Free 1B3 IF, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • AKT2 Antibody | 1B3 thumbnail image 1
                • AKT2 Antibody | 1B3 thumbnail image 2
                • AKT2 Antibody | 1B3 thumbnail image 3

              Write your review

              You may also be interested in...