AKT1 (pThr450) Antibody

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AKT1 (pThr450) Antibody gallery image 1

Western blot analysis of extracts from HEK-293 cells using Rabbit anti AKT1 (pThr450) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with either Insulin (100nM) for 10 minutes or EGF (100ng/ml) for 30 minutes

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  • Rabbit anti AKT1 (pThr450)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2546WBdatasheet pdfdatasheet pdf50 µl
    AHP2546
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti AKT1 (pThr450) antibody recognizes AKT1, also known as RAC-alpha serine/threonine-protein kinase, protein kinase B alpha or Proto-oncogene c-Akt, when phosphorylated at threonine 450. AKT1 is a 480 amino acid ~48 kDa protein kinase that play a key role in the PI3K-Akt signaling pathway, known to regulate cell growth, proliferation and survival. Phosphorylation of threonine 308 and serine 473 are required for full activation of AKT1. Newly synthesized Akt is suggested to require phosphorylation at threonine 450 for proper protein folding (Liao and Hung 2010).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/5001/1000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
    • Instructions For Use

    Additional AKT1 (pThr450) Antibody Formats

    Formats Applications Sizes available
    AKT1 (pThr450) Antibody : Purified WB 50 µl
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
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          Recommended Positive Controls

            Histology Controls

              Further Reading

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