AKT1 Antibody | AbD03813

AKT1 Antibody | AbD03813 gallery image 1

Western blot analysis of MCF-7 human adenocarcinoma whole cell lysate probed with Human anti Human AKT1 antibody (HCA017) followed by HRP conjugated F(ab')2Goat anti Human IgG, visualized by chemiluminescence

AKT1 Antibody | AbD03813 gallery image 2

Sandwich ELISA analysis of human AKT1 expression using Human anti Human AKT1 (HCA017) as a capture reagent and biotinylated Mouse anti Human AKT1 (MCA4779Z) as a detection reagent with recombinant human AKT1 as antigen to generate the standard curve.Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum samples, undiluted (green and blue) and plasma samples, undiluted (orange and red) are indicated

  • AKT1 Antibody | AbD03813 thumbnail image 1
  • AKT1 Antibody | AbD03813 thumbnail image 2
  • Human anti AKT1
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
    HuCAL Fab bivalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA017E, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    • Human anti AKT1 antibody, clone AbD03813 recognizes AKT1, it does not react with human AKT2 or AKT3.
      Two serine/threonine kinases, designated AKT1 and AKT2 exhibit sequence homology with the protein kinase A and C families and are encoded by the c-AKT proto-oncogene. AKT1 and the related AKT2 are activated by platelet-derived growth factor (PDGF). The activation is rapid and specific, and it is abrogated by mutations in the Pleckstrin homology domain (PH) of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. Additionally, the activation of AKT1 and AKT2 is inhibited by the PI kinase inhibitor wortmannin. Taken together, this data strongly suggests that the protein signals downstream of the PI kinases. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
      Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 is dephosphorylated by PHLPP.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA2 ug/ml
      Western Blotting2 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional AKT1 Antibody Formats

    Formats Clone Applications Sizes available
    AKT1 Antibody : Purified AbD03813 E, WB 0.1 mg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Human IgG F(ab')2:HRP0500-00990.5 mlE, WB
      Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
      Mouse anti c-MycMCA22001 mgC, E, F*, P, WB*

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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