AKT1 Antibody | AbD02147

AKT1 Antibody | AbD02147 gallery image 1

A. Ponceau staining of protein kinases: Lanes 1: AKT1 2: AKT2 3: AKT3 4: TSF-1 5: CSK. B. Western Blot analysis showing a single band with AKT1 when probed with 2ug/ml HCA003 followed by Goat anti Human IgG F(ab')2: HRP (0500-0099)

AKT1 Antibody | AbD02147 gallery image 2

Sandwich ELISA analysis of human AKT1 expression using Human anti Human AKT1 (HCA003) as a capture reagent and biotinylated Mouse anti Human AKT1 (MCA4779Z) as a detection reagent with recombinant human AKT1 as antigen to generate the standard curve.Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum samples, undiluted (green and blue) and plasma samples, undiluted (orange and red) are indicated

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  • Human anti AKT1
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    • Product Type
      Monoclonal Antibody
    • Isotype
      HuCAL Fab bivalent
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA003E, WBdatasheet pdfdatasheet pdf0.1 mg
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
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      • Human anti Human AKT1 antibody, clone AbD02147 recognizes AKT1, a cytoplasmic kinase which is expressed in the nucleus after activation by integrin-linked protein kinase 1 (ILK1). Clone AbD02147 does not bind to human AKT2 and AKT3 (81% and 82% identical to AKT1, respectively).

        Two serine/threonine kinases, designated AKT1 and AKT2 exhibit sequence homology with the protein kinase A and C families and are encoded by the c-AKT proto-oncogene. AKT1 and the related AKT2 are activated by platelet-derived growth factor (PDGF). The activation is rapid and specific, and it is abrogated by mutations in the Pleckstrin homology domain (PH) of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. Additionally, the activation of AKT1 and AKT2 is inhibited by the PI kinase inhibitor wortmannin. Taken together, this data strongly suggests that the protein signals downstream of the PI kinases. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
        Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 is dephosphorylated by PHLPP.
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting2ug/ml

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • ELISA
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional AKT1 Antibody Formats

      Formats Clone Applications Sizes available
      AKT1 Antibody : Purified AbD02147 E, WB 0.1 mg

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Human IgG F(ab')2:HRP0500-00990.5 mlE, WB
        Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
        Mouse anti c-MycMCA22001 mgC, E, F*, P, WB*

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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