AKR1C1 Antibody (PrecisionAbTM Antibody)

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AKR1C1 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with AKR1C1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 6.4 second exposure. Arrow points to AKR1C1 (molecular weight 36 kDa)

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  • AKR1C1 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • AKR1C1 Antibody with Control Lysate
  • AKR1C1 Antibody with Control Lysate
  • Mouse anti AKR1C1
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    AKR1C1 antibody recognizes aldo-keto reductase family 1 member C1 also known as 20-alpha-hydroxysteroid dehydrogenase, chlordecone reductase homolog HAKRC, high-affinity hepatic bile acid-binding protein or HBAB. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00334WBdatasheet pdfdatasheet pdf100 µl
      VMA00334
      VMA00334KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VMA00334K
      VMA00334KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00334KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Mouse anti Human AKR1C1 antibody detects the aldo-keto reductase family 1 member C1, also known as 20-alpha-hydroxysteroid dehydrogenase (20-alpha-HSD), chlordecone reductase homolog HAKRC and high-affinity hepatic bile acid-binding protein (HBAB). Encoded by the AKR1C1 gene, aldo-keto reductase family 1 member C1 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. Aldo-keto reductase family 1 member C1 catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. In the liver and intestine, it may have a role in the transport of bile, monitoring the intrahepatic bile acid concentration.

        Mouse anti Human AKR1C1 antibody detects a band of 36 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti AKR1C1 detects a band of approximately 36 kDa in A549 cell lysates
        Anti AKR1C1 detects a band of approximately 36 kDa in A549 cell lysates
        Anti AKR1C1 detects a band of approximately 36 kDa in A549 cell lysates
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G
        Mouse monoclonal antibody prepared by affinity chromatography on Protein G
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Recombinant human AKR1C1
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg A549 lysate lyophilized in RIPA buffer.
        400μg A549 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        androsterone dehydrogenase (B-specific) activity
        aldo-keto reductase activity
        cytosol
        oxidation-reduction process
        bile acid binding
        intestinal cholesterol absorption
        indanol dehydrogenase activity
        trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity
        cholesterol homeostasis
        protein homooligomerization
        bile acid metabolic process
        17-alpha,20-alpha-dihydroxypregn-4-en-3-one dehydrogenase activity
        bile acid and bile salt transport
        response to organophosphorus
        xenobiotic metabolic process
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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