AKR1A1 Antibody (PrecisionAbTM Antibody) | OTI9F1

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AKR1A1 Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI9F1 gallery image 1

Western blot analysis of whole cell lysates probed with AKR1A1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 50 second exposure. Arrow points to AKR1A1 (molecular weight 38 kDa)

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  • AKR1A1 Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI9F1 thumbnail image 1
  • Mouse anti AKR1A1
  • AKR1A1 Antibody with Control Lysate
  • AKR1A1 Antibody with Control Lysate
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    AKR1A1 antibody recognizes alcohol dehydrogenase [NADP(+)], also known as HEL-S-165mP, alcohol dehydrogenase, aldehyde reductase, aldo-keto reductase family 1 member A1, dihydrodiol dehydrogenase 3, epididymis secretory protein Li 6 or epididymis secretory sperm binding protein Li 165mP. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG2a
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00109WBdatasheet pdfdatasheet pdf100 µl
      VMA00109
      VMA00109KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VMA00109K
      VMA00109KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00109KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Mouse anti Human AKR1A1 antibody recognizes AKR1A1, also known as HEL-S-165mP, alcohol dehydrogenase, alcohol dehydrogenase [NADP(+)], aldehyde reductase, aldo-keto reductase family 1 member A1, dihydrodiol dehydrogenase 3, epididymis secretory protein Li 6 and epididymis secretory sperm binding protein Li 165mP.

        The AKR1A1 gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member, also known as aldehyde reductase, is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue. Multiple alternatively spliced transcript variants of AKR1A1 exist, all encoding the same protein (provided by RefSeq, Jan 2011).

        Mouse anti Human AKR1A1 antibody detects a band of 38 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti AKR1A1 detects a band of approximately 38 kDa in LNCaP cell lysates.
        Anti AKR1A1 detects a band of approximately 38 kDa in LNCaP cell lysates.
        Anti AKR1A1 detects a band of approximately 38 kDa in LNCaP cell lysates.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Mouse monoclonal antibody purified by affinity chromatography from ascites.
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
      • Immunogen
        Full length recombinant human AKR1A1 (NP_006057) produced in HEK293T cells
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg LNCaP lysate lyophilized in RIPA buffer.
        400μg LNCaP lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        oxidation-reduction process
        electron carrier activity
        protein binding
        aldehyde reductase activity
        glucose metabolic process
        alcohol dehydrogenase (NADP+) activity
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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