AK1 Antibody | 3A6-1F5

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AK1 Antibody | 3A6-1F5 gallery image 1

Immunofluorescence showing AK1 in HeLa cells using Mouse anti Human AK1:Azide Free (MCA2918Z)

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AK1 Antibody | 3A6-1F5 gallery image 2

Western blot analysis of AK1 expression in HeLa using Mouse anti Human AK1:Azide Free (MCA2918Z)

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  • Mouse anti Human AK1:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    3A6-1F5
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2918ZIF, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA2918Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human AK1 antibody, clone 3A6-1F5 recognizes human Adenylate kinase isoenzyme 1, also known as AK1, ATP-AMP transphosphorylase 1, ATP:AMP phosphotransferase, Adenylate monophosphate kinase or Myokinase. AK1 is a 194 amino acid ~22 kDa cytoplasmic phosphatatse regulatinfg the reversible transfer of the terminal phosphate group from ATP to AMP.

      Mutation of the AK1 gene can lead to the hereditary condition “Hemolytic anemia due to adenylate kinase deficiency (HAAKD), characterized by hemolytic anemia and undetectable erythrocyte adenylate kinase activity.characterized by hemolytic anemia and undetectable erythrocyte adenylate kinase activity (UniProt: P00568).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 1 - 195 of human AK1.
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      ATP metabolic process
      adenylate kinase activity
      cytosol
      nucleobase, nucleoside and nucleotide interconversion
      ATP binding
      protein binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence0.110 ug/ml
      Western Blotting0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional AK1 Antibody Formats

    Formats Clone Applications Sizes available
    AK1 Antibody : Preservative Free 3A6-1F5 IF, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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