AIF Antibody

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AIF Antibody gallery image 1

Western blot analysis of AIF in K562 cell lysate probed with Rabbit anti Human AIF antibody (AHP579)

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AIF Antibody gallery image 2

Immunocytochemical staining of Jurkat Tcell lymphoblast like cells with Rabbit anti Human AIF (AHP579)

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  • AIF Antibody thumbnail image 1
  • AIF Antibody thumbnail image 2
  • Rabbit anti Human AIF (aa109-122)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP579WB*datasheet pdfdatasheet pdf0.1 mg
    AHP579
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Human AIF antibody recognizes human apoptosis-inducing factor 1, mitochondrial, also known as AIF or programmed cell death protein 8. Human AIF is a 613 amino acid mitochondrial protein bearing a 54 amino acid N-terminal transit peptide and a 47 amino acid propeptide region adjacent. The mature peptide is a 512 amino acid ~67 kDa mitochondrial intermembrane space protein formed during apoptosis. Rabbit anti Human AIF antibody reacts with the N-terminal region of the mature protein. AIF is widely expressed in human tissues and is released to the cytoplasm and then translocates to the nucleus on certain death signals where it is involved in the induction of nuclear apoptosis (Kim et al. 2006).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to AIF (NT) were raised by repeated immunisation of rabbit with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
    • Preservative Stabilisers
      0.02%Sodium Azide
    • Immunogen
      Peptide corresponding to amino acids 109 - 122 of human AIF (NT).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting(1)1 / 500
      (1)
      AHP579 is suitable for western blotting under non-reducing conditions only.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional AIF Antibody Formats

    Formats Applications Sizes available
    AIF Antibody : Purified WB* 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Further Reading

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