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AID Antibody | EK2-5G9

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  • Rat anti Human AID
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4756GTE, F *, P *, WBdatasheet pdfdatasheet pdf50 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rat anti Human AID antibody, clone EK2-5G9 recognizes human Single-stranded DNA cytosine deaminase, also known as Activation-induced cytidine deaminase or Cytidine aminohydrolase. AID is a 198 amino acid ~24 kDa RNA-editing enzyme essential for the initiation of immunoglobulin class switch recombination, somatic hypermutation and gene conversion.

      AID is selectively expressed by germinal centre B cells and B cell-derived lymphomas (Basso et al. 2012). Mutation in the AICDA gene is responsible for the autosomal recessive condition Immunodeficiency with hyper-IgM 2 (HIGM2) A rare immunodeficiency syndrome characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE resulting in extreme susceptibility to bacterial infection.
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Synthetic peptide corresponding to amino acids 185-198 of human AID.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from Long Evans rats were fused with cells of the P3X63-Ag8.653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      cytidine deaminase activity
      B cell differentiation
      zinc ion binding
      protein binding
      mRNA processing
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting
      Flow Cytometry(1)
      Immunohistology - Paraffin(2)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. EDTA pH8.0 is recommended for this purpose.
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Germinal centre B cells
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional AID Antibody Formats

    Formats Clone Applications Sizes available
    AID Antibody : Purified EK2-5G9 E, F *, P *, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2b Negative ControlMCA6006GA0.1 mgF

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Germinal centre B cells

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