Aggrecan Antibody | 7E1

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Aggrecan Antibody | 7E1 gallery image 1

Published customer image:
Mouse anti Human aggrecan antibody, clone 7E1 (MCA1451G) used for the detection of aggrecan expressing cells in murine micromass cultures by immunocytochemistry.
Image caption:
Regulation of Extracellular matrix (ECM) composition by Bone morphogenetic protein (BMP) family growth factors during chondrogenesis. Mouse micromass cultures without treatment (A–E), Gdf-5 treatment (F–J), and Bmp-7 treatment (K–O); Alcian blue stain (A–K) showed cartilage nodules, Immunohistochemical stain for type 1 collagen (A, F, K), type II collagen (C, H, M), aggrecan (D, I, N), and fibronectin (E, J, O). Statistical analysis for histochemical and immunohistochemical data, (P) = percentage of positive area for Alcian blue, type I collagen, type II collagen, aggrecan, and fibronectin stains in micromasses with different treatments; mean values are shown with Standard deviations (SD) (n = three independent experiments), One-way Analysis of variance (ANOVA). ***P <0.0001; **p <0.001; *p <0.05). Gdf-5 mainly induces aggrecan expression, type II collagen, and fibronectin; aggrecan is an articular cartilage marker; in contrast, Bmp-7 induce type I collagen, an extracellular component of hypertrophic cartilage and bone.

From: Garciadiego-Cázares D, Aguirre-Sánchez HI, Abarca-Buis RF, Kouri JB, Velasquillo C, Ibarra C (2015) Regulation of α5 and αV Integrin Expression by GDF-5 and BMP-7 in Chondrocyte Differentiation and Osteoarthritis.
PLoS ONE 10(5): e0127166.

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  • Aggrecan Antibody | 7E1 thumbnail image 1
  • Mouse anti Human Aggrecan
  • Mouse anti Human Aggrecan
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    7E1
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1451GC, E, IP, WBdatasheet pdfdatasheet pdf0.2 mg
    MCA1451G
    MCA1451TCdatasheet pdfdatasheet pdf0.2 ml
    MCA1451T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human aggrecan antibody, clone 7E1 recognizes human aggrecan, a proteoglycan and member of the aggrecan/versican proteoglycan family, which forms a major component of the extracellular matrix (ECM) of both cartilage and the central nervous system (CNS).

      Studies using a panel of core protein-directed antibodies against human aggrecan, revealed the distribution of different aggrecan isoforms within the CNS, and sub-divided the isoforms into clusters 1-5 accordingly. These studies also showed a difference in the relative abundance of these isoforms when comparing brain and cartilage tissues (Virgintino et al. 2009).

      Mouse anti Human aggrecan antibody, clone 7E1 recognizes a ~205 kDa trypsin derived fragment of aggrecan.

      Note: Originally described as being of the isotype IgM, clone 7E1 was found to be unstable and was re-cloned by the originator and the new stable sub-clone was confirmed as being an IgG1.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Bovineyes
      Chickenno
      Fishno
      Ratno
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Tissue Culture Supernatant - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      10mMSodium Azide
      0.09%Sodium Azide
    • Immunogen
      Purified human articular cartilage aggrecan.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch
    • GO Terms
      proteinaceous extracellular matrix
      proteolysis
      skeletal system development
      extracellular matrix structural constituent
      sugar binding
      hyaluronic acid binding
      protein binding
      cell adhesion
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Aggrecan Antibody Formats

    Formats Clone Applications Sizes available
    Aggrecan Antibody : S/N 7E1 C 0.2 ml
    Aggrecan Antibody : Purified 7E1 C, E, IP, WB 0.2 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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